Roles of two homotetrameric kinesins in sea urchin embryonic cell division

Kitty K. Chui; Gregory C. Rogers; Anna M. Kashina; Karen P. Wedaman; David J. Sharp; Duy T. Nguyen; Fred Wilt; Jonathan M. Scholey

doi:10.1074/jbc.M005948200

Roles of two homotetrameric kinesins in sea urchin embryonic cell division

Kitty K. Chui, Gregory C. Rogers, Anna M. Kashina, Karen P. Wedaman, David J. Sharp, Duy T. Nguyen, Fred Wilt, Jonathan M. Scholey

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

To improve our understanding of the roles of microtubule cross-linking motors in mitosis, we analyzed two sea urchin embryonic kinesin-related proteins. It is striking to note that both of these proteins behave as homotetramers, but one behaves as a more compact molecule than the other. These observations suggest that these two presumptive motors could cross-link microtubules into bundles with different spacing. Both motors localize to mitotic spindles, and antibody microinjection experiments suggest that they have mitotic functions. Thus, one of these kinesin-related proteins may cross-link spindle microtubules into loose bundles that are 'tightened' by the other.

Original language	English (US)
Pages (from-to)	38005-38011
Number of pages	7
Journal	Journal of Biological Chemistry
Volume	275
Issue number	48
DOIs	https://doi.org/10.1074/jbc.M005948200
State	Published - Dec 1 2000
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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title = "Roles of two homotetrameric kinesins in sea urchin embryonic cell division",

abstract = "To improve our understanding of the roles of microtubule cross-linking motors in mitosis, we analyzed two sea urchin embryonic kinesin-related proteins. It is striking to note that both of these proteins behave as homotetramers, but one behaves as a more compact molecule than the other. These observations suggest that these two presumptive motors could cross-link microtubules into bundles with different spacing. Both motors localize to mitotic spindles, and antibody microinjection experiments suggest that they have mitotic functions. Thus, one of these kinesin-related proteins may cross-link spindle microtubules into loose bundles that are 'tightened' by the other.",

author = "Chui, {Kitty K.} and Rogers, {Gregory C.} and Kashina, {Anna M.} and Wedaman, {Karen P.} and Sharp, {David J.} and Nguyen, {Duy T.} and Fred Wilt and Scholey, {Jonathan M.}",

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AU - Chui, Kitty K.

AU - Rogers, Gregory C.

AU - Kashina, Anna M.

AU - Wedaman, Karen P.

AU - Sharp, David J.

AU - Nguyen, Duy T.

AU - Wilt, Fred

AU - Scholey, Jonathan M.

PY - 2000/12/1

Y1 - 2000/12/1

N2 - To improve our understanding of the roles of microtubule cross-linking motors in mitosis, we analyzed two sea urchin embryonic kinesin-related proteins. It is striking to note that both of these proteins behave as homotetramers, but one behaves as a more compact molecule than the other. These observations suggest that these two presumptive motors could cross-link microtubules into bundles with different spacing. Both motors localize to mitotic spindles, and antibody microinjection experiments suggest that they have mitotic functions. Thus, one of these kinesin-related proteins may cross-link spindle microtubules into loose bundles that are 'tightened' by the other.

AB - To improve our understanding of the roles of microtubule cross-linking motors in mitosis, we analyzed two sea urchin embryonic kinesin-related proteins. It is striking to note that both of these proteins behave as homotetramers, but one behaves as a more compact molecule than the other. These observations suggest that these two presumptive motors could cross-link microtubules into bundles with different spacing. Both motors localize to mitotic spindles, and antibody microinjection experiments suggest that they have mitotic functions. Thus, one of these kinesin-related proteins may cross-link spindle microtubules into loose bundles that are 'tightened' by the other.

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Roles of two homotetrameric kinesins in sea urchin embryonic cell division

Abstract

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