Roles of O-Fucose Glycans in Notch Signaling Revealed by Mutant Mice

Linchao Lu, Pamela Stanley

Research output: Contribution to journalReview article

17 Scopus citations

Abstract

Notch receptor signaling is important for many developmental processes in the metazoa. Insights into how Notch receptor signaling is regulated have been obtained from the characterization of mutants of model organisms in which Notch signaling is perturbed. Here we describe the effects of mutations that alter the glycosylation of Notch receptors and Notch ligands in the mouse. The extracellular domain of Notch receptors and Notch ligands carries N-glycans and O-glycans, including O-fucose and O-glucose glycans. Mutations in several genes that inhibit the synthesis of O-fucose glycans, and one that also affects the maturation of N-glycans, cause Notch signaling defects and disrupt development.

Original languageEnglish (US)
Pages (from-to)127-136
Number of pages10
JournalMethods in enzymology
Volume417
DOIs
StatePublished - Nov 27 2006
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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