Roles of C/EBP class bZip proteins in the growth and cell competition of Rp ('Minute') mutants in Drosophila

Jorge Blanco, Jacob C. Cooper, Nicholas E. Baker

Research output: Contribution to journalArticle

Abstract

Reduced copy number of ribosomal protein (Rp) genes adversely affects both flies and mammals. Xrp1 encodes a reportedly Drosophila-specific AT-hook, bZIP protein responsible for many of the effects including the elimination of Rp mutant cells by competition with wild type cells. Irbp18, an evolutionarily conserved bZIP gene, heterodimerizes with Xrp1 and with another bZip protein, dATF4. We show that Irbp18 is required for the effects of Xrp1, whereas dATF4 does not share the same phenotype, indicating that Xrp1/Irbp18 is the complex active in Rp mutant cells, independently of other complexes that share Irbp18. Xrp1 and Irbp18 transcripts and proteins are upregulated in Rp mutant cells by auto-regulatory expression that depends on the Xrp1 DNA binding domains and is necessary for cell competition. We show that Xrp1 is conserved beyond Drosophila, although under positive selection for rapid evolution, and that at least one human bZip protein can similarly affect Drosophila development.

Original languageEnglish (US)
JournaleLife
Volume9
DOIs
StatePublished - Jan 7 2020

Fingerprint

Ribosomal Proteins
Drosophila
Growth
Proteins
Genes
Basic-Leucine Zipper Transcription Factors
AT-Hook Motifs
Mammals
Hooks
Diptera
DNA
Phenotype

Keywords

  • bZip domain
  • c/ebp
  • cell competition
  • D. melanogaster
  • developmental biology
  • growth control
  • ribosomal protein
  • xrp1

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Roles of C/EBP class bZip proteins in the growth and cell competition of Rp ('Minute') mutants in Drosophila. / Blanco, Jorge; Cooper, Jacob C.; Baker, Nicholas E.

In: eLife, Vol. 9, 07.01.2020.

Research output: Contribution to journalArticle

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