Roles for N- and O-glycans in early mouse development

Suzannah A. Williams, Pamela Stanley

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

Glycosylation is the most abundant posttranslational protein modification. Specific glycans covalently attached to glycoproteins contribute to their functions, ensuring appropriate folding, secretion, half-life, and receptor-ligand interactions [1]. Many different classes of glycans exist, but those discussed herein are the complex and hybrid N-glycans, core 1-derived O-glycans, and O-linked fucose glycans. The synthesis of each class of glycan is initiated by the addition of a single sugar, or group of sugars, to certain amino acids or amino acid sequons by specific glycosyltransferases via a particular linkage. The subsequent sugars are added individually in a carefully orchestrated pathway by specific glycosyltransferases that reside in the secretory compartments of the cell. Thus, the glycans ultimately synthesized by a cell depend on the cohort of glycosyltransferases, nucleotide sugar synthases, and transporters expressed by that cell, which will be influenced by metabolic state and stage of development. To determine roles for complex and hybrid N-glycans, core 1-derived O-glycans, and O-fucose glycans (Fig. 20.1) in oogenesis, fertilization, blastogenesis, implantation, and embryonic development, we used a maternal and zygotic gene-targeting approach.

Original languageEnglish (US)
Title of host publicationThe Molecular Immunology of Complex Carbohydrates-3
EditorsAlbert Wu
Pages397-410
Number of pages14
DOIs
StatePublished - Dec 19 2011

Publication series

NameAdvances in Experimental Medicine and Biology
Volume705
ISSN (Print)0065-2598

Keywords

  • Conditional gene targeting
  • Fertilization
  • N-glycans
  • O-glycans
  • Stage-specific embryonic antigens

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Williams, S. A., & Stanley, P. (2011). Roles for N- and O-glycans in early mouse development. In A. Wu (Ed.), The Molecular Immunology of Complex Carbohydrates-3 (pp. 397-410). (Advances in Experimental Medicine and Biology; Vol. 705). https://doi.org/10.1007/978-1-4419-7877-6_20