Roles for N- and O-glycans in early mouse development

Suzannah A. Williams, Pamela Stanley

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Glycosylation is the most abundant posttranslational protein modification. Specific glycans covalently attached to glycoproteins contribute to their functions, ensuring appropriate folding, secretion, half-life, and receptor-ligand interactions [1]. Many different classes of glycans exist, but those discussed herein are the complex and hybrid N-glycans, core 1-derived O-glycans, and O-linked fucose glycans. The synthesis of each class of glycan is initiated by the addition of a single sugar, or group of sugars, to certain amino acids or amino acid sequons by specific glycosyltransferases via a particular linkage. The subsequent sugars are added individually in a carefully orchestrated pathway by specific glycosyltransferases that reside in the secretory compartments of the cell. Thus, the glycans ultimately synthesized by a cell depend on the cohort of glycosyltransferases, nucleotide sugar synthases, and transporters expressed by that cell, which will be influenced by metabolic state and stage of development. To determine roles for complex and hybrid N-glycans, core 1-derived O-glycans, and O-fucose glycans (Fig. 20.1) in oogenesis, fertilization, blastogenesis, implantation, and embryonic development, we used a maternal and zygotic gene-targeting approach.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
Pages397-410
Number of pages14
Volume705
DOIs
StatePublished - 2011

Publication series

NameAdvances in Experimental Medicine and Biology
Volume705
ISSN (Print)00652598

Fingerprint

Polysaccharides
Sugars
Glycosyltransferases
Fucose
Glycosylation
Amino Acids
Oogenesis
Gene Targeting
Post Translational Protein Processing
Lymphocyte Activation
Fertilization
Embryonic Development
Half-Life
Glycoproteins
Nucleotides
Genes
Mothers
Ligands

Keywords

  • Conditional gene targeting
  • Fertilization
  • N-glycans
  • O-glycans
  • Stage-specific embryonic antigens

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Williams, S. A., & Stanley, P. (2011). Roles for N- and O-glycans in early mouse development. In Advances in Experimental Medicine and Biology (Vol. 705, pp. 397-410). (Advances in Experimental Medicine and Biology; Vol. 705). https://doi.org/10.1007/978-1-4419-7877-6_20

Roles for N- and O-glycans in early mouse development. / Williams, Suzannah A.; Stanley, Pamela.

Advances in Experimental Medicine and Biology. Vol. 705 2011. p. 397-410 (Advances in Experimental Medicine and Biology; Vol. 705).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Williams, SA & Stanley, P 2011, Roles for N- and O-glycans in early mouse development. in Advances in Experimental Medicine and Biology. vol. 705, Advances in Experimental Medicine and Biology, vol. 705, pp. 397-410. https://doi.org/10.1007/978-1-4419-7877-6_20
Williams SA, Stanley P. Roles for N- and O-glycans in early mouse development. In Advances in Experimental Medicine and Biology. Vol. 705. 2011. p. 397-410. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-1-4419-7877-6_20
Williams, Suzannah A. ; Stanley, Pamela. / Roles for N- and O-glycans in early mouse development. Advances in Experimental Medicine and Biology. Vol. 705 2011. pp. 397-410 (Advances in Experimental Medicine and Biology).
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