Role of the Tsc1-Tsc2 complex in signaling and transport across the cell membrane in the fission yeast Schizosaccharomyces pombe

Sanae Matsumoto, Amitabha Bandyopadhyay, David J. Kwiatkowski, Umadas Maitra, Tomohiro Matsumoto

Research output: Contribution to journalArticle

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Abstract

Heterozygous inactivation of either human TSC1 or TSC2 causes tuberous sclerosis (TSC), in which development of benign tumors, hamartomas, occurs via a two-hit mechanism. In this study, fission yeast genes homologous to TSC1 and TSC2 were identified, and their protein products were shown to physically interact like the human gene products. Strains lacking tsc1+ or tsc2+ were defective in uptake of nutrients from the environment. An amino acid permease, which is normally positioned on the plasma membrane, aggregated in the cytoplasm or was confined in vacuole-like structures in Δtsc1 and Δtsc2 strains. Deletion of tsc1+ or tsc2+ also caused a defect in conjugation. When a limited number of the cells were mixed, they conjugated poorly. The conjugation efficiency was improved by increased cell density. Δtsc1 cells were not responsive to a mating pheromone, P-factor, suggesting that Tsc1 has an important role in the signal cascade for conjugation. These results indicate that the fission yeast Tsc1-Tsc2 complex plays a role in the regulation of protein trafficking and suggest a similar function for the human proteins. We also show that fission yeast Int6 is involved in a similar process, but functions in an independent genetic pathway.

Original languageEnglish (US)
Pages (from-to)1053-1063
Number of pages11
JournalGenetics
Volume161
Issue number3
StatePublished - 2002

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Schizosaccharomyces
Cell Membrane
Cell Count
Amino Acid Transport Systems
Tuberous Sclerosis
Hamartoma
Protein Transport
Vacuoles
Genes
Cytoplasm
Proteins
Food
Neoplasms

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Matsumoto, S., Bandyopadhyay, A., Kwiatkowski, D. J., Maitra, U., & Matsumoto, T. (2002). Role of the Tsc1-Tsc2 complex in signaling and transport across the cell membrane in the fission yeast Schizosaccharomyces pombe. Genetics, 161(3), 1053-1063.

Role of the Tsc1-Tsc2 complex in signaling and transport across the cell membrane in the fission yeast Schizosaccharomyces pombe. / Matsumoto, Sanae; Bandyopadhyay, Amitabha; Kwiatkowski, David J.; Maitra, Umadas; Matsumoto, Tomohiro.

In: Genetics, Vol. 161, No. 3, 2002, p. 1053-1063.

Research output: Contribution to journalArticle

Matsumoto, S, Bandyopadhyay, A, Kwiatkowski, DJ, Maitra, U & Matsumoto, T 2002, 'Role of the Tsc1-Tsc2 complex in signaling and transport across the cell membrane in the fission yeast Schizosaccharomyces pombe', Genetics, vol. 161, no. 3, pp. 1053-1063.
Matsumoto S, Bandyopadhyay A, Kwiatkowski DJ, Maitra U, Matsumoto T. Role of the Tsc1-Tsc2 complex in signaling and transport across the cell membrane in the fission yeast Schizosaccharomyces pombe. Genetics. 2002;161(3):1053-1063.
Matsumoto, Sanae ; Bandyopadhyay, Amitabha ; Kwiatkowski, David J. ; Maitra, Umadas ; Matsumoto, Tomohiro. / Role of the Tsc1-Tsc2 complex in signaling and transport across the cell membrane in the fission yeast Schizosaccharomyces pombe. In: Genetics. 2002 ; Vol. 161, No. 3. pp. 1053-1063.
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