Role of the second and third intracellular loops of metabotropic glutamate receptors in mediating dual signal transduction activation

Anna Francesconi, Robert M. Duvoisin

Research output: Contribution to journalArticle

131 Scopus citations

Abstract

On the basis of sequence homology and structural similarities, metabotropic glutamate receptors (mGluRs), extracellular Ca2+-sensing receptor, γ-aminobutyric acid type B receptor, and pheromone receptors are enlisted in a distinct family within the larger G protein-coupled receptor superfamily. When expressed in heterologous systems, group I mGluRs can activate dual signal transduction pathways, phosphoinositides turnover and cAMP production. To investigate the structural basis of these coupling properties, we introduced single amino acid substitutions within the second and third intracellular loops (i2 and i3) of mGluR1α. Wild-type and mutant receptors were expressed in human embryonic kidney 293 cells and analyzed for their capacity to stimulate both signaling cascades. Each domain appeared to be critical for the coupling to phospholipase C and adenylyl cyclase. Within i2, Thr695, Lys697, and Ser702 were found to be selectively involved in the interaction with G(q) class α subunit(s), whereas mutation of Pro695 and the deletion Cys694-Thr695 affected only G(s) coupling. Furthermore, the mutation K690A profoundly altered mGluR1α signaling properties and imparted to the receptor the ability to couple to the inhibitory cAMP pathway. Within i3, we uncovered two residues, Arg775 and Phe781, that are crucial for coupling to both pathways, since their substitution leads to receptor inactivation.

Original languageEnglish (US)
Pages (from-to)5615-5624
Number of pages10
JournalJournal of Biological Chemistry
Volume273
Issue number10
DOIs
StatePublished - Mar 6 1998
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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