Role of rRAB22b, an oligodendrocyte protein, in regulation of transport of vesicles from trans Golgi to endocytic compartments

A. G. Rodriguez-Gabin, M. Cammer, G. Almazan, Maureen J. Charron, J. N. Larocca

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Intracellular membrane trafficking plays an essential role in the biogenesis and maintenance of myelin. Members of the Rab protein family are important components of the systems that regulate intracellular vesicle transport. We examine the function of rRab22b, a novel rat Rab protein cloned from an oligodendrocyte cDNA library, by visualizing and identifying in living Hela cells the organelles that contain rRab22b. Our results show that rRab22b is present in the trans Golgi/TGN and endocytic compartments. Trafficking of membranes from trans Golgi to endocytic compartments takes place via small tubulo vesicular organelles containing rRab22b. The formation of vesicles in the trans Golgi also appears to be regulated by rRab22b. Additionally, our results suggest that rRab22b controls the transport of vesicles from the trans Golgi to endocytic compartments that localize in oligodendrocyte processes. That rRab22b is involved in the transport of certain proteins from trans Golgi to myelin is suggested by the evidence that certain proteins being targeted to the plasma membrane are first transported from trans Golgi to endocytic compartments.

Original languageEnglish (US)
Pages (from-to)1149-1160
Number of pages12
JournalJournal of Neuroscience Research
Volume66
Issue number6
DOIs
StatePublished - Dec 15 2001

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Transport Vesicles
Oligodendroglia
Myelin Sheath
Organelles
Intracellular Membranes
Proteins
Gene Library
HeLa Cells
Carrier Proteins
Maintenance
Cell Membrane
Membranes

Keywords

  • Endosomes
  • Myelin proteins
  • Oligodendrocytes
  • Rab proteins
  • Signaling, myelin
  • Trans Golgi
  • Vesicle transport

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Role of rRAB22b, an oligodendrocyte protein, in regulation of transport of vesicles from trans Golgi to endocytic compartments. / Rodriguez-Gabin, A. G.; Cammer, M.; Almazan, G.; Charron, Maureen J.; Larocca, J. N.

In: Journal of Neuroscience Research, Vol. 66, No. 6, 15.12.2001, p. 1149-1160.

Research output: Contribution to journalArticle

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