Role of Peritoneal Mesothelial cells and fibroblasts in the synthesis of hyaluronan during peritoneal dialysis

Andrzej Breborowicz, Justyna Wisniewska, Alicja Polubinska, Katarzyna Wieczorowska-Tobis, Leo Martis, Dimitrios G. Oreopoulos

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Objective: To assess the in vitro synthesis rate of hyaluronan (HA) by human peritoneal mesothelial cells and peritoneal fibroblasts in the presence of effluent dialysate from continuous ambulatory peritoneal dialysis (CAPD) patients. Methods: We used primary cultures of human peritoneal mesothelial cells and peritoneal fibroblasts from nonuremic patients to study the effect of interleukin-1β (II-1β) and pooled effluent dialysate, from noninfected and infected CAPD patients, on the synthesis of HA by the studied cells. We also tested the effect of the exogenous HA on the synthesis rate of that glycosaminoglycan. We studied the correlation between HA concentration in effluent dialysate and the stimulatory effect of that solution on in vitro synthesis of HA by mesothelium. Results: Peritoneal fibroblasts produce more HA than mesothelial cells. Noninfected effluent dialysates or dialysates from CAPD patients with peritonitis stimulate synthesis of HA by mesothelial cells and fibroblasts. Interleukin-1β has a stimulating effect, which was synergistic with effluent dialysates, on the synthesis of HA by mesothelium and peritoneal fibroblasts. A weak correlation was demonstrated between the level of HA in effluent dialysate and the stimulatory effect of that dialysate on in vitro synthesis of HA by mesothelial cells. Conclusions: Peritoneal fibroblasts are a more potent source of HA than are mesothelial cells, but probably the latter are the main source of HA in drained dialysate. Although effluent dialysates contain factors that stimulate the production of HA by mesothelium, there is weak correlation between that stimulatory effect and the actual HA concentration in the dialysate, which, in some patients, might suggest low 'responsiveness' of the membrane.

Original languageEnglish (US)
Pages (from-to)382-386
Number of pages5
JournalPeritoneal Dialysis International
Volume18
Issue number4
DOIs
StatePublished - 1998
Externally publishedYes

Keywords

  • Fibroblasts
  • Hyaluronan
  • Mesothelial cells

ASJC Scopus subject areas

  • Nephrology

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