TY - JOUR
T1 - Role of no synthase in the development of Trypanosoma craz/-induced cardiomyopathy in mice
AU - Durand, Jorge L.
AU - Mukherjee, Shankar
AU - Commodari, Fernando
AU - De Souza, Andrea P.
AU - Zhao, Dazhi
AU - Machado, Fabiana S.
AU - Tanowitz, Herbert B.
AU - Jelicks, Linda A.
PY - 2009/5
Y1 - 2009/5
N2 - Trypanosoma cruzi infection results in an increase in myocardial NO and intense inflammation. NO modu-lates the T. craz/-induced myocardial inflammatory reaction. NO synthase (NOS)l-, NOS2-, and NOS3-null mice were infected with T. cruzi (Brazil strain). Infected NOSl-null mice had increased parasitemia, mortality, and left ventricular inner diameter (LVID). Chronically infected NOS1-and NOS2-null and wild-type mice (WT) exhibited increased right ventricular internal diameter (RVID), although the fold increase in the NOS2-null mice was smaller. Infected NOS3-null mice exhibited a significant reduction both in LVID and RVID. Reverse transcriptase-polymerase chain reaction showed expression of NOS2 and NOS3 in hearts of infected NOS1-null and WT mice, whereas infected NOS2-null hearts showed little change in expression of other NOS isoforms. Infected NOS3-null hearts showed an increase only in NOS1 expres-sion. These results may indicate different roles for NOS isoforms in T. cruzi-induced cardiomyopathy.
AB - Trypanosoma cruzi infection results in an increase in myocardial NO and intense inflammation. NO modu-lates the T. craz/-induced myocardial inflammatory reaction. NO synthase (NOS)l-, NOS2-, and NOS3-null mice were infected with T. cruzi (Brazil strain). Infected NOSl-null mice had increased parasitemia, mortality, and left ventricular inner diameter (LVID). Chronically infected NOS1-and NOS2-null and wild-type mice (WT) exhibited increased right ventricular internal diameter (RVID), although the fold increase in the NOS2-null mice was smaller. Infected NOS3-null mice exhibited a significant reduction both in LVID and RVID. Reverse transcriptase-polymerase chain reaction showed expression of NOS2 and NOS3 in hearts of infected NOS1-null and WT mice, whereas infected NOS2-null hearts showed little change in expression of other NOS isoforms. Infected NOS3-null hearts showed an increase only in NOS1 expres-sion. These results may indicate different roles for NOS isoforms in T. cruzi-induced cardiomyopathy.
UR - http://www.scopus.com/inward/record.url?scp=66149158195&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=66149158195&partnerID=8YFLogxK
U2 - 10.4269/ajtmh.2009.80.782
DO - 10.4269/ajtmh.2009.80.782
M3 - Article
C2 - 19407124
AN - SCOPUS:66149158195
SN - 0002-9637
VL - 80
SP - 782
EP - 787
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 5
ER -