Role of no synthase in the development of Trypanosoma craz/-induced cardiomyopathy in mice

Jorge L. Durand, Shankar Mukherjee, Fernando Commodari, Andrea P. De Souza, Dazhi Zhao, Fabiana S. Machado, Herbert B. Tanowitz, Linda A. Jelicks

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Trypanosoma cruzi infection results in an increase in myocardial NO and intense inflammation. NO modu-lates the T. craz/-induced myocardial inflammatory reaction. NO synthase (NOS)l-, NOS2-, and NOS3-null mice were infected with T. cruzi (Brazil strain). Infected NOSl-null mice had increased parasitemia, mortality, and left ventricular inner diameter (LVID). Chronically infected NOS1-and NOS2-null and wild-type mice (WT) exhibited increased right ventricular internal diameter (RVID), although the fold increase in the NOS2-null mice was smaller. Infected NOS3-null mice exhibited a significant reduction both in LVID and RVID. Reverse transcriptase-polymerase chain reaction showed expression of NOS2 and NOS3 in hearts of infected NOS1-null and WT mice, whereas infected NOS2-null hearts showed little change in expression of other NOS isoforms. Infected NOS3-null hearts showed an increase only in NOS1 expres-sion. These results may indicate different roles for NOS isoforms in T. cruzi-induced cardiomyopathy.

Original languageEnglish (US)
Pages (from-to)782-787
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume80
Issue number5
StatePublished - May 2009

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Trypanosoma
Cardiomyopathies
Trypanosoma cruzi
Nitric Oxide Synthase
Protein Isoforms
Parasitemia
Reverse Transcriptase Polymerase Chain Reaction
Brazil
Inflammation
Mortality
Infection

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology
  • Medicine(all)

Cite this

Durand, J. L., Mukherjee, S., Commodari, F., De Souza, A. P., Zhao, D., Machado, F. S., ... Jelicks, L. A. (2009). Role of no synthase in the development of Trypanosoma craz/-induced cardiomyopathy in mice. American Journal of Tropical Medicine and Hygiene, 80(5), 782-787.

Role of no synthase in the development of Trypanosoma craz/-induced cardiomyopathy in mice. / Durand, Jorge L.; Mukherjee, Shankar; Commodari, Fernando; De Souza, Andrea P.; Zhao, Dazhi; Machado, Fabiana S.; Tanowitz, Herbert B.; Jelicks, Linda A.

In: American Journal of Tropical Medicine and Hygiene, Vol. 80, No. 5, 05.2009, p. 782-787.

Research output: Contribution to journalArticle

Durand, JL, Mukherjee, S, Commodari, F, De Souza, AP, Zhao, D, Machado, FS, Tanowitz, HB & Jelicks, LA 2009, 'Role of no synthase in the development of Trypanosoma craz/-induced cardiomyopathy in mice', American Journal of Tropical Medicine and Hygiene, vol. 80, no. 5, pp. 782-787.
Durand JL, Mukherjee S, Commodari F, De Souza AP, Zhao D, Machado FS et al. Role of no synthase in the development of Trypanosoma craz/-induced cardiomyopathy in mice. American Journal of Tropical Medicine and Hygiene. 2009 May;80(5):782-787.
Durand, Jorge L. ; Mukherjee, Shankar ; Commodari, Fernando ; De Souza, Andrea P. ; Zhao, Dazhi ; Machado, Fabiana S. ; Tanowitz, Herbert B. ; Jelicks, Linda A. / Role of no synthase in the development of Trypanosoma craz/-induced cardiomyopathy in mice. In: American Journal of Tropical Medicine and Hygiene. 2009 ; Vol. 80, No. 5. pp. 782-787.
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