Abstract
Trypanosoma cruzi infection results in an increase in myocardial NO and intense inflammation. NO modu-lates the T. craz/-induced myocardial inflammatory reaction. NO synthase (NOS)l-, NOS2-, and NOS3-null mice were infected with T. cruzi (Brazil strain). Infected NOSl-null mice had increased parasitemia, mortality, and left ventricular inner diameter (LVID). Chronically infected NOS1-and NOS2-null and wild-type mice (WT) exhibited increased right ventricular internal diameter (RVID), although the fold increase in the NOS2-null mice was smaller. Infected NOS3-null mice exhibited a significant reduction both in LVID and RVID. Reverse transcriptase-polymerase chain reaction showed expression of NOS2 and NOS3 in hearts of infected NOS1-null and WT mice, whereas infected NOS2-null hearts showed little change in expression of other NOS isoforms. Infected NOS3-null hearts showed an increase only in NOS1 expres-sion. These results may indicate different roles for NOS isoforms in T. cruzi-induced cardiomyopathy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 782-787 |
| Number of pages | 6 |
| Journal | American Journal of Tropical Medicine and Hygiene |
| Volume | 80 |
| Issue number | 5 |
| State | Published - May 2009 |
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ASJC Scopus subject areas
- Parasitology
- Infectious Diseases
- Virology
- Medicine(all)
Cite this
Role of no synthase in the development of Trypanosoma craz/-induced cardiomyopathy in mice. / Durand, Jorge L.; Mukherjee, Shankar; Commodari, Fernando; De Souza, Andrea P.; Zhao, Dazhi; Machado, Fabiana S.; Tanowitz, Herbert B.; Jelicks, Linda A.
In: American Journal of Tropical Medicine and Hygiene, Vol. 80, No. 5, 05.2009, p. 782-787.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Role of no synthase in the development of Trypanosoma craz/-induced cardiomyopathy in mice
AU - Durand, Jorge L.
AU - Mukherjee, Shankar
AU - Commodari, Fernando
AU - De Souza, Andrea P.
AU - Zhao, Dazhi
AU - Machado, Fabiana S.
AU - Tanowitz, Herbert B.
AU - Jelicks, Linda A.
PY - 2009/5
Y1 - 2009/5
N2 - Trypanosoma cruzi infection results in an increase in myocardial NO and intense inflammation. NO modu-lates the T. craz/-induced myocardial inflammatory reaction. NO synthase (NOS)l-, NOS2-, and NOS3-null mice were infected with T. cruzi (Brazil strain). Infected NOSl-null mice had increased parasitemia, mortality, and left ventricular inner diameter (LVID). Chronically infected NOS1-and NOS2-null and wild-type mice (WT) exhibited increased right ventricular internal diameter (RVID), although the fold increase in the NOS2-null mice was smaller. Infected NOS3-null mice exhibited a significant reduction both in LVID and RVID. Reverse transcriptase-polymerase chain reaction showed expression of NOS2 and NOS3 in hearts of infected NOS1-null and WT mice, whereas infected NOS2-null hearts showed little change in expression of other NOS isoforms. Infected NOS3-null hearts showed an increase only in NOS1 expres-sion. These results may indicate different roles for NOS isoforms in T. cruzi-induced cardiomyopathy.
AB - Trypanosoma cruzi infection results in an increase in myocardial NO and intense inflammation. NO modu-lates the T. craz/-induced myocardial inflammatory reaction. NO synthase (NOS)l-, NOS2-, and NOS3-null mice were infected with T. cruzi (Brazil strain). Infected NOSl-null mice had increased parasitemia, mortality, and left ventricular inner diameter (LVID). Chronically infected NOS1-and NOS2-null and wild-type mice (WT) exhibited increased right ventricular internal diameter (RVID), although the fold increase in the NOS2-null mice was smaller. Infected NOS3-null mice exhibited a significant reduction both in LVID and RVID. Reverse transcriptase-polymerase chain reaction showed expression of NOS2 and NOS3 in hearts of infected NOS1-null and WT mice, whereas infected NOS2-null hearts showed little change in expression of other NOS isoforms. Infected NOS3-null hearts showed an increase only in NOS1 expres-sion. These results may indicate different roles for NOS isoforms in T. cruzi-induced cardiomyopathy.
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M3 - Article
C2 - 19407124
AN - SCOPUS:66149158195
VL - 80
SP - 782
EP - 787
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
SN - 0002-9637
IS - 5
ER -