Role of macrophages and colony-stimulating factor-1 in murine antiglomerular basement membrane glomerulonephritis

Joel Neugarten, G. W. Feith, K. J M Assmann, Z. Shan, E. R. Stanley, D. Schlondorff

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Macrophages have been shown to mediate glomerular injury in antiglomerular basement membrane (anti-GBM) glomerulonephritis in rats and rabbits. To evaluate the role of macrophages and the macrophage-related cytokines, colony stimulating factor-1 (CSF-1), monocyte chemoattractant protein-1 (MCP-1) and RANTES, accelerated anti-GBM nephritis was studied in op/op mutant mice, which lack CSF-1 and are severely macrophage deficient, and in heterozygous op/+ control mice. Observations were made 24 h and 3 days after the injection of rabbit anti-mouse GBM antibody in mice preimmunized with rabbit immunoglobulin G. Proteinuria rose progressively in both groups but did not differ between them (urine protein/creatinine ratio at 3 days: 1.01 ± 0.38 in op/op versus 1.45 ± 0.43 in op/+; P, not significant). In both op/op and op/+ mice, anti-GBM nephritis was associated with renal expression of mRNA for RANTES and MCP-1 and barely detectable levels of mRNA for GSF-1. In contrast, these cytokines were not expressed in sham-injected mice. Morphologic lesions appeared earlier in op/op mice but were comparable by Day 3. Glomerular injury consisted of capillary thrombosis and endothelial cell damage associated with mild to moderate leukocyte infiltration. Despite enhanced expression of mRNA for RANTES and MCP-1, glomerular macrophage infiltration was not increased in op/+ mice. It was concluded that, in mice, in contrast to rats and rabbits, accelerated anti-GBM nephritis may develop in the absence of both CSF-1 and macrophage infiltration.

Original languageEnglish (US)
Pages (from-to)1903-1909
Number of pages7
JournalJournal of the American Society of Nephrology
Volume5
Issue number11
StatePublished - 1995

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Macrophage Colony-Stimulating Factor
Glomerulonephritis
Basement Membrane
Macrophages
Chemokine CCL5
Nephritis
Chemokine CCL2
Rabbits
Messenger RNA
Cytokines
Wounds and Injuries
Proteinuria
Creatinine
Thrombosis
Leukocytes
Endothelial Cells
Immunoglobulin G
Urine
Kidney
Injections

Keywords

  • antiglomerular basement membrane nephritis
  • colony stimulating factor-1
  • experimental glomerulonephritis
  • macrophages

ASJC Scopus subject areas

  • Nephrology

Cite this

Role of macrophages and colony-stimulating factor-1 in murine antiglomerular basement membrane glomerulonephritis. / Neugarten, Joel; Feith, G. W.; Assmann, K. J M; Shan, Z.; Stanley, E. R.; Schlondorff, D.

In: Journal of the American Society of Nephrology, Vol. 5, No. 11, 1995, p. 1903-1909.

Research output: Contribution to journalArticle

Neugarten, Joel ; Feith, G. W. ; Assmann, K. J M ; Shan, Z. ; Stanley, E. R. ; Schlondorff, D. / Role of macrophages and colony-stimulating factor-1 in murine antiglomerular basement membrane glomerulonephritis. In: Journal of the American Society of Nephrology. 1995 ; Vol. 5, No. 11. pp. 1903-1909.
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