Role of endothelial selectins in wound repair

Meera Subramaniam, Simin Saffaripour, Livingston Van De Water, Paul S. Frenette, Tanya N. Mayadas, Richard O. Hynes, Denisa D. Wagner

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

P- and E-selectins are adhesion molecules expressed on activated endothelium and platelets at sites of vascular injury and inflammation. The selectins are important for leukocyte recruitment. Because little is known about the role of selectins in wound healing, we studied cutaneous wound repair of full-thickness excisional skin wounds in mice lacking P-selectin, E-selectin, or both of these selectins. The absence of either selectin alone had no notable effect on healing, and the only deficit observed was a delay in early neutrophil extravasation in the P-selectin-deficient mice. Mice deficient in both P- and E-selectins had markedly reduced recruitment of inflammatory cells and impaired closure of the wounds. Wound sections, studied up to 3 days after wounding, showed significant impairment of neutrophil influx. Macrophage numbers were also reduced in the double mutants at 3 and 7 days after wounding as compared with wild-type mice. Additionally, a wider epithelial gap in the wounds of the P- and E-selectin- double-deficient mice 3 days after wounding indicated delayed keratinocyte migration. These results demonstrate an important combined role for P- and E-selectins in processes leading to wound healing.

Original languageEnglish (US)
Pages (from-to)1701-1709
Number of pages9
JournalAmerican Journal of Pathology
Volume150
Issue number5
StatePublished - 1997
Externally publishedYes

Fingerprint

Selectins
P-Selectin
E-Selectin
Wounds and Injuries
Wound Healing
Neutrophils
Skin
Vascular System Injuries
Keratinocytes
Endothelium
Leukocytes
Blood Platelets
Macrophages
Inflammation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Subramaniam, M., Saffaripour, S., Van De Water, L., Frenette, P. S., Mayadas, T. N., Hynes, R. O., & Wagner, D. D. (1997). Role of endothelial selectins in wound repair. American Journal of Pathology, 150(5), 1701-1709.

Role of endothelial selectins in wound repair. / Subramaniam, Meera; Saffaripour, Simin; Van De Water, Livingston; Frenette, Paul S.; Mayadas, Tanya N.; Hynes, Richard O.; Wagner, Denisa D.

In: American Journal of Pathology, Vol. 150, No. 5, 1997, p. 1701-1709.

Research output: Contribution to journalArticle

Subramaniam, M, Saffaripour, S, Van De Water, L, Frenette, PS, Mayadas, TN, Hynes, RO & Wagner, DD 1997, 'Role of endothelial selectins in wound repair', American Journal of Pathology, vol. 150, no. 5, pp. 1701-1709.
Subramaniam M, Saffaripour S, Van De Water L, Frenette PS, Mayadas TN, Hynes RO et al. Role of endothelial selectins in wound repair. American Journal of Pathology. 1997;150(5):1701-1709.
Subramaniam, Meera ; Saffaripour, Simin ; Van De Water, Livingston ; Frenette, Paul S. ; Mayadas, Tanya N. ; Hynes, Richard O. ; Wagner, Denisa D. / Role of endothelial selectins in wound repair. In: American Journal of Pathology. 1997 ; Vol. 150, No. 5. pp. 1701-1709.
@article{ce01549b13bc4c2b9e4005d16ae09aeb,
title = "Role of endothelial selectins in wound repair",
abstract = "P- and E-selectins are adhesion molecules expressed on activated endothelium and platelets at sites of vascular injury and inflammation. The selectins are important for leukocyte recruitment. Because little is known about the role of selectins in wound healing, we studied cutaneous wound repair of full-thickness excisional skin wounds in mice lacking P-selectin, E-selectin, or both of these selectins. The absence of either selectin alone had no notable effect on healing, and the only deficit observed was a delay in early neutrophil extravasation in the P-selectin-deficient mice. Mice deficient in both P- and E-selectins had markedly reduced recruitment of inflammatory cells and impaired closure of the wounds. Wound sections, studied up to 3 days after wounding, showed significant impairment of neutrophil influx. Macrophage numbers were also reduced in the double mutants at 3 and 7 days after wounding as compared with wild-type mice. Additionally, a wider epithelial gap in the wounds of the P- and E-selectin- double-deficient mice 3 days after wounding indicated delayed keratinocyte migration. These results demonstrate an important combined role for P- and E-selectins in processes leading to wound healing.",
author = "Meera Subramaniam and Simin Saffaripour and {Van De Water}, Livingston and Frenette, {Paul S.} and Mayadas, {Tanya N.} and Hynes, {Richard O.} and Wagner, {Denisa D.}",
year = "1997",
language = "English (US)",
volume = "150",
pages = "1701--1709",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Role of endothelial selectins in wound repair

AU - Subramaniam, Meera

AU - Saffaripour, Simin

AU - Van De Water, Livingston

AU - Frenette, Paul S.

AU - Mayadas, Tanya N.

AU - Hynes, Richard O.

AU - Wagner, Denisa D.

PY - 1997

Y1 - 1997

N2 - P- and E-selectins are adhesion molecules expressed on activated endothelium and platelets at sites of vascular injury and inflammation. The selectins are important for leukocyte recruitment. Because little is known about the role of selectins in wound healing, we studied cutaneous wound repair of full-thickness excisional skin wounds in mice lacking P-selectin, E-selectin, or both of these selectins. The absence of either selectin alone had no notable effect on healing, and the only deficit observed was a delay in early neutrophil extravasation in the P-selectin-deficient mice. Mice deficient in both P- and E-selectins had markedly reduced recruitment of inflammatory cells and impaired closure of the wounds. Wound sections, studied up to 3 days after wounding, showed significant impairment of neutrophil influx. Macrophage numbers were also reduced in the double mutants at 3 and 7 days after wounding as compared with wild-type mice. Additionally, a wider epithelial gap in the wounds of the P- and E-selectin- double-deficient mice 3 days after wounding indicated delayed keratinocyte migration. These results demonstrate an important combined role for P- and E-selectins in processes leading to wound healing.

AB - P- and E-selectins are adhesion molecules expressed on activated endothelium and platelets at sites of vascular injury and inflammation. The selectins are important for leukocyte recruitment. Because little is known about the role of selectins in wound healing, we studied cutaneous wound repair of full-thickness excisional skin wounds in mice lacking P-selectin, E-selectin, or both of these selectins. The absence of either selectin alone had no notable effect on healing, and the only deficit observed was a delay in early neutrophil extravasation in the P-selectin-deficient mice. Mice deficient in both P- and E-selectins had markedly reduced recruitment of inflammatory cells and impaired closure of the wounds. Wound sections, studied up to 3 days after wounding, showed significant impairment of neutrophil influx. Macrophage numbers were also reduced in the double mutants at 3 and 7 days after wounding as compared with wild-type mice. Additionally, a wider epithelial gap in the wounds of the P- and E-selectin- double-deficient mice 3 days after wounding indicated delayed keratinocyte migration. These results demonstrate an important combined role for P- and E-selectins in processes leading to wound healing.

UR - http://www.scopus.com/inward/record.url?scp=0030999872&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030999872&partnerID=8YFLogxK

M3 - Article

C2 - 9137094

AN - SCOPUS:0030999872

VL - 150

SP - 1701

EP - 1709

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 5

ER -