Role of copper ion in regulating ligand binding in a myoglobin-based cytochrome c oxidase model

Changyuan Lu, Xuan Zhao, Yi Lu, Denis L. Rousseau, Syun-Ru Yeh

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Cytochrome c oxidase (CcO), the terminal enzyme in the mitochondrial respiratory chain, catalyzes the four-electron reduction of dioxygen to water in a binuclear center comprised of a high-spin heme (heme a3) and a copper atom (CuB) coordinated by three histidine residues. As a minimum model for CcO, a mutant of sperm whale myoglobin, named Cu BMb, has been engineered, in which a copper atom is held in the distal heme pocket by the native E7 histidine and two nonnative histidine residues. In this work, the role of the copper in regulating ligand binding in CuBMb was investigated. Resonance Raman studies show that the presence of copper in CO-bound CuBMb leads to a CcO-like distal heme pocket. Stopped-flow data show that, upon the initiation of the CO binding reaction, the ligand first binds to the Cu+; it subsequently transfers from Cu+ to Fe2+ in an intramolecular process, similar to that reported for CcO. The high CO affinity toward Cu+ and the slow intramolecular CO transfer rate between Cu+ and Fe2+ in the CuBMb/Cu + complex are analogous to those in Thermus thermophilus CcO (TtCcO) but distinct from those in bovine CcO (bCcO). Additional kinetic studies show that, upon photolysis of the NO-bound CuBMb/Cu+ complex, the photolyzed ligand transiently binds to Cu+ and subsequently rebinds to Fe2+, accounting for the 100% geminate recombination yield, similar to that found in TtCcO. The data demonstrate that the Cu BMb/Cu+ complex reproduces essential structural and kinetic features of CcO and that the complex is more akin to TtCcO than to bCcO.

Original languageEnglish (US)
Pages (from-to)1598-1605
Number of pages8
JournalJournal of the American Chemical Society
Volume132
Issue number5
DOIs
StatePublished - Feb 10 2010

Fingerprint

Myoglobin
Electron Transport Complex IV
Copper
Ligands
Ions
Carbon Monoxide
Thermus thermophilus
Heme
Histidine
Sperm Whale
Atoms
Kinetics
Photolysis
Electron Transport
Genetic Recombination
Enzymes
Electrons
Oxygen
Water

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Role of copper ion in regulating ligand binding in a myoglobin-based cytochrome c oxidase model. / Lu, Changyuan; Zhao, Xuan; Lu, Yi; Rousseau, Denis L.; Yeh, Syun-Ru.

In: Journal of the American Chemical Society, Vol. 132, No. 5, 10.02.2010, p. 1598-1605.

Research output: Contribution to journalArticle

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abstract = "Cytochrome c oxidase (CcO), the terminal enzyme in the mitochondrial respiratory chain, catalyzes the four-electron reduction of dioxygen to water in a binuclear center comprised of a high-spin heme (heme a3) and a copper atom (CuB) coordinated by three histidine residues. As a minimum model for CcO, a mutant of sperm whale myoglobin, named Cu BMb, has been engineered, in which a copper atom is held in the distal heme pocket by the native E7 histidine and two nonnative histidine residues. In this work, the role of the copper in regulating ligand binding in CuBMb was investigated. Resonance Raman studies show that the presence of copper in CO-bound CuBMb leads to a CcO-like distal heme pocket. Stopped-flow data show that, upon the initiation of the CO binding reaction, the ligand first binds to the Cu+; it subsequently transfers from Cu+ to Fe2+ in an intramolecular process, similar to that reported for CcO. The high CO affinity toward Cu+ and the slow intramolecular CO transfer rate between Cu+ and Fe2+ in the CuBMb/Cu + complex are analogous to those in Thermus thermophilus CcO (TtCcO) but distinct from those in bovine CcO (bCcO). Additional kinetic studies show that, upon photolysis of the NO-bound CuBMb/Cu+ complex, the photolyzed ligand transiently binds to Cu+ and subsequently rebinds to Fe2+, accounting for the 100{\%} geminate recombination yield, similar to that found in TtCcO. The data demonstrate that the Cu BMb/Cu+ complex reproduces essential structural and kinetic features of CcO and that the complex is more akin to TtCcO than to bCcO.",
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