Role of cofilin in epidermal growth factor-stimulated actin polymerization and lamellipod protrusion

Amanda Y. Chan, Maryse Bailly, Noureddine Zebda, Jeffrey E. Segall, John S. Condeelis

Research output: Contribution to journalArticle

201 Citations (Scopus)

Abstract

Stimulation of metastatic MTLn3 cells with epidermal growth factor (EGF) causes a rapid and transient increase in actin nucleation activity resulting from the appearance of free barbed ends at the extreme leading edge of extending lamellipods. To investigate the role of cofilin in EGF-stimulated actin polymerization and lamellipod extension in MTLn3 cells, we examined in detail the temporal and spatial distribution of cofilin relative to free barbed ends and characterized the actin dynamics by measuring the changes in the number of actin filaments. EGF stimulation triggers a transient increase in cofilin in the leading edge near the membrane, which is precisely cotemporal with the appearance of free barbed ends there. A deoxyribonuclease I binding assay shows that the number of filaments per cell increases by 1.5-fold after EGF stimulation. Detection of pointed ends in situ using deoxyribonuclease I binding demonstrates that this increase in the number of pointed ends is confined to the leading edge compartment, and does not occur within stress fibers or in the general cytoplasm. Using a light microscope severing assay, cofilin's severing activity was observed directly in cell extracts and shown to be activated after stimulation of the cells with EGF. Microinjection of function-blocking antibodies against cofilin inhibits the appearance of free barbed ends at the leading edge and lamellipod protrusion after EGF stimulation. These results support a model in which EGF stimulation recruits cofilin to the leading edge where its severing activity is activated, leading to the generation of short actin filaments with free barbed ends that participate in the nucleation of actin polymerization.

Original languageEnglish (US)
Pages (from-to)531-542
Number of pages12
JournalJournal of Cell Biology
Volume148
Issue number3
DOIs
StatePublished - Feb 7 2000

Fingerprint

Actin Depolymerizing Factors
Epidermal Growth Factor
Polymerization
Actins
Deoxyribonuclease I
Actin Cytoskeleton
Stress Fibers
Blocking Antibodies
Microinjections
Cell Extracts
Cytoplasm
Light
Membranes

Keywords

  • Actin-related protein (ARP) 2/3
  • Chemotaxis
  • F-actin severing
  • Metastasis
  • Motility

ASJC Scopus subject areas

  • Cell Biology

Cite this

Role of cofilin in epidermal growth factor-stimulated actin polymerization and lamellipod protrusion. / Chan, Amanda Y.; Bailly, Maryse; Zebda, Noureddine; Segall, Jeffrey E.; Condeelis, John S.

In: Journal of Cell Biology, Vol. 148, No. 3, 07.02.2000, p. 531-542.

Research output: Contribution to journalArticle

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