Role of Choline in the Modulation of Degenerative Processes: In Vivo and in Vitro Studies

Tania Merinas-Amo, Inmaculada Tasset, Antonio M. DÍaz-Carretero, Ángeles Alonso-Moraga, Fernando Calahorro

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The purpose of the present study was to examine the nutraceutical potential of choline as an added value to its well-known brain nutrient role. Several toxicity, antitoxicity, genotoxicity, antigenotoxicity, and longevity endpoints were checked in the somatic mutation and recombination test in in vivo Drosophila animal model. Cytotoxicity in human leukemia-60 cell line (HL-60) promyelocytic and NIH3T3 mouse fibroblast cells, proapoptotic DNA fragmentation, comet assay, methylation status, and macroautophagy (MA) activity were tested in in vitro assays. Choline is not only safe but it is also able to protect against the DNA damage caused by an oxidative genotoxin. Moreover, it improves the life extension in the animal model. The in vitro results show that it is able to exhibit genetic damage against leukemia HL-60 cells. Single-strand breaks in DNA are observed at the molecular level in treatments with choline, although only a significant hypermethylation on the long interspersed elements-1 and a hypomethylation on the satellite-alpha DNA repetitive DNA sequences of HL-60 cells at the lowest concentration (0.447 mM) were observed. Besides, choline decreased MA at the lower assayed concentration and the MA response to topoisomerase inhibitor (etoposide) is maintained in the presence of treatment with 0.22 mM choline. Taking into account the hopeful results obtained in the in vivo and in vitro assays, choline could be proposed as a substance with an important nutraceutical value for different purposes.

Original languageEnglish (US)
Pages (from-to)223-234
Number of pages12
JournalJournal of Medicinal Food
Volume20
Issue number3
DOIs
StatePublished - Mar 1 2017
Externally publishedYes

Fingerprint

Choline
HL-60 Cells
Autophagy
Dietary Supplements
Leukemia
Animal Models
Topoisomerase Inhibitors
Satellite DNA
Single-Stranded DNA Breaks
Comet Assay
Nucleic Acid Repetitive Sequences
Mutagens
DNA Fragmentation
Etoposide
Life Expectancy
Methylation
Genetic Recombination
DNA Damage
Drosophila
In Vitro Techniques

Keywords

  • Antigenotoxicity
  • Antitoxicity
  • Choline
  • Cytotoxicity
  • DNA damage
  • Drosophila melanogaster
  • HL-60 and NIH3T3 cell lines
  • Longevity
  • Macroautophagy
  • Methylation status

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Role of Choline in the Modulation of Degenerative Processes : In Vivo and in Vitro Studies. / Merinas-Amo, Tania; Tasset, Inmaculada; DÍaz-Carretero, Antonio M.; Alonso-Moraga, Ángeles; Calahorro, Fernando.

In: Journal of Medicinal Food, Vol. 20, No. 3, 01.03.2017, p. 223-234.

Research output: Contribution to journalArticle

Merinas-Amo, T, Tasset, I, DÍaz-Carretero, AM, Alonso-Moraga, Á & Calahorro, F 2017, 'Role of Choline in the Modulation of Degenerative Processes: In Vivo and in Vitro Studies', Journal of Medicinal Food, vol. 20, no. 3, pp. 223-234. https://doi.org/10.1089/jmf.2016.0075
Merinas-Amo, Tania ; Tasset, Inmaculada ; DÍaz-Carretero, Antonio M. ; Alonso-Moraga, Ángeles ; Calahorro, Fernando. / Role of Choline in the Modulation of Degenerative Processes : In Vivo and in Vitro Studies. In: Journal of Medicinal Food. 2017 ; Vol. 20, No. 3. pp. 223-234.
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