Role for CD14, TLR2, and TLR4 in bacterial product-induced anorexia

C. Von Meyenburg, B. H. Hrupka, D. Arsenijevic, Gary J. Schwartz, R. Landmann, W. Langhans

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The cell surface component CD14 and the toll-like receptors 2 and 4 (TLR2 and TLR4) are important in mediating the immune responses to bacterial products in mammals. Using mice genetically deficient in CD14, TLR2, or TLR4, we studied the role of these molecules in the anorectic effects of LPS and muramyl dipeptide (MDP). CD14 or TLR2 knockout (KO) and TLR4-deficient (TLR4-DEF) mice as well as corresponding wild-type (WT) colittermates were injected intraperitoneally at dark onset with LPS (2 μg/mouse), MDP (10 mg/kg), interleukin-1β (IL-1β, 150 ng/mouse), or vehicle, and food intake was recorded. LPS and MDP reduced food intake in WT mice of all genotypes tested. The anorectic effect of LPS was attenuated (P < 0.04) in CD14-KO and TLR4-DEF mice but not in TLR2-KO (P > 0.05). The anorectic effect of MDP was blunted in CD14-KO and TLR2-KO (P < 0.02) mice but not in TLR4-DEF mice. IL-1> reduced food intake similarly in all genotypes tested. These results indicate that CD14 is involved in mediating the anorectic effects of both LPS and MDP. Furthermore, TLR4 and TLR2 are specifically involved in mediating the anorectic effects of LPS and MDP, respectively. The results are consistent with the hypothesis that TLR4 functions as the true LPS receptor and that TLR2 is involved in recognition of gram-positive bacterial products.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume287
Issue number2 56-2
DOIs
StatePublished - Aug 2004
Externally publishedYes

Fingerprint

Acetylmuramyl-Alanyl-Isoglutamine
Anorexia
Appetite Depressants
Eating
Interleukin-1
Genotype
CD14 Antigens
Toll-Like Receptor 2
Toll-Like Receptor 4
Cellular Structures
Mammals

Keywords

  • Cytokines
  • Food intake
  • Interleukin-1
  • Lipopolysaccharide
  • Muramyl dipeptide
  • Toll-like receptor 2 and 4

ASJC Scopus subject areas

  • Physiology

Cite this

Role for CD14, TLR2, and TLR4 in bacterial product-induced anorexia. / Von Meyenburg, C.; Hrupka, B. H.; Arsenijevic, D.; Schwartz, Gary J.; Landmann, R.; Langhans, W.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 287, No. 2 56-2, 08.2004.

Research output: Contribution to journalArticle

Von Meyenburg, C. ; Hrupka, B. H. ; Arsenijevic, D. ; Schwartz, Gary J. ; Landmann, R. ; Langhans, W. / Role for CD14, TLR2, and TLR4 in bacterial product-induced anorexia. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2004 ; Vol. 287, No. 2 56-2.
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