@article{e48775a646754cfdb30278e27644809a,
title = "Rodent hair is a Poor biomarker for internal manganese exposure",
abstract = "Hair is used as a biomarker of manganese (Mn) exposure, yet there is limited evidence to support its utility to quantify internal vs external Mn exposure. C57BL/6 J mice and Sprague-Dawley rats were exposed in two blocks of 3 subcutaneous injections every 3 days starting on day 0 or 20. The control group received two blocks of saline (vehicle); Treatment A received the first block as Mn (50 mg/kg MnCl2 tetrahydrate), with the second block as either methylmercury (MeHg at 2.6 or 1.3 mg/kg) for mice or vehicle for rats; and Treatment B received Mn for both blocks. Hair was collected on days 0 and 60 from all treatment groups and Mn quantified by inductively coupled plasma-mass spectrometry (ICP-MS) and total Hg by Direct Mercury Analyzer (DMA). No correlation between internal Mn dose and hair Mn was observed, whereas hair Hg was significantly elevated in MeHg exposed vs non-exposed mice. Whole body Mn content at day 60 was quantified postmortem by neutron activation analysis, which detected significantly elevated Mn for Treatment B in mice and rats. Overall, we find no evidence to support the use of hair as a valid biomarker for internal exposure to Mn at a neurotoxic level.",
keywords = "Biomarkers, Hair, Manganese, Metals, Methylmercury, Neutron activation analysis",
author = "Balachandran, {Rekha C.} and Yanko, {Frank M.} and Pinjing Cheng and Prince, {Lisa M.} and Rivers, {Chloe N.} and Patricia Morcillo and Akinyemi, {Ayodele J.} and Sana Tabbassum and Pfalzer, {Anna C.} and Nie, {Linda H.} and Michael Aschner and Bowman, {Aaron B.}",
note = "Funding Information: We would like to thank the International Manganese Institute (IMnI) for partial funding support of this project. We thank Arlene Rothwell of the Purdue Chemistry Department Research Instrumentation Center (RIC) for assistance in ICP-MS data collection. We would also like to thank the Purdue University Centrally Managed Animal Facility Care Staff for housing and managing the mice used in this project. We greatly appreciate the technical assistance of Bingying Han and Grace Fawoye. Funding Information: We would like to thank the International Manganese Institute (IMnI) for partial funding support of this project. We thank Arlene Rothwell of the Purdue Chemistry Department Research Instrumentation Center (RIC) for assistance in ICP-MS data collection. We would also like to thank the Purdue University Centrally Managed Animal Facility Care Staff for housing and managing the mice used in this project. We greatly appreciate the technical assistance of Bingying Han and Grace Fawoye. Publisher Copyright: {\textcopyright} 2021 Elsevier Ltd",
year = "2021",
month = nov,
doi = "10.1016/j.fct.2021.112555",
language = "English (US)",
volume = "157",
journal = "Food and Chemical Toxicology",
issn = "0278-6915",
publisher = "Elsevier Limited",
}