RNASeq in C. elegans following manganese exposure

Nancy L. Parmalee, Shahina B. Maqbool, Bin Ye, Brent Calder, Aaron B. Bowman, Michael Aschner

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Manganese is a metal that is required for optimal biological functioning of organisms. Absorption, cellular import and export, and excretion of manganese are all tightly regulated. While some genes involved in regulation, such as DMT-1 and ferroportin, are known, it is presumed that many more are involved and as yet unknown. Excessive exposure to manganese, usually in industrial settings such as mining or welding, can lead to neurotoxicity and a condition known as manganism that closely resembles Parkinson's disease. Elucidating transcriptional changes following manganese exposure could lead to the development of biomarkers for exposure. This unit presents a protocol for RNA sequencing in the worm Caenorhabditis elegans to assay for transcriptional changes following exposure to manganese. This protocol is adaptable to any environmental exposure in C. elegans. The protocol results in counts of gene transcripts in control versus exposed conditions and a ranked list of differentially expressed genes for further study.

Original languageEnglish (US)
Pages (from-to)11.20.1-11.20.17
JournalCurrent Protocols in Toxicology
Volume2015
DOIs
StatePublished - 2015

Fingerprint

Manganese
Genes
Caenorhabditis elegans
RNA Sequence Analysis
Welding
Environmental Exposure
Biomarkers
Parkinson Disease
Assays
Metals
RNA

Keywords

  • C. elegans
  • Manganese
  • RNASeq

ASJC Scopus subject areas

  • Toxicology

Cite this

RNASeq in C. elegans following manganese exposure. / Parmalee, Nancy L.; Maqbool, Shahina B.; Ye, Bin; Calder, Brent; Bowman, Aaron B.; Aschner, Michael.

In: Current Protocols in Toxicology, Vol. 2015, 2015, p. 11.20.1-11.20.17.

Research output: Contribution to journalArticle

Parmalee, Nancy L. ; Maqbool, Shahina B. ; Ye, Bin ; Calder, Brent ; Bowman, Aaron B. ; Aschner, Michael. / RNASeq in C. elegans following manganese exposure. In: Current Protocols in Toxicology. 2015 ; Vol. 2015. pp. 11.20.1-11.20.17.
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