RNase H overproduction allows the expression of stress-induced genes in the absence of topoisomerase I

Bokun Cheng; Shan Rui; Chengling Ji; Vivien W. Gong; Tina K. Van Dyk; Marc Drolet; Yuk Ching Tse-Dinh

doi:10.1016/S0378-1097(03)00209-X

RNase H overproduction allows the expression of stress-induced genes in the absence of topoisomerase I

Bokun Cheng, Shan Rui, Chengling Ji, Vivien W. Gong, Tina K. Van Dyk, Marc Drolet, Yuk Ching Tse-Dinh

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Induction of stress proteins in response to heat shock was found to be reduced significantly in Escherichia coli with ΔtopA mutation. RNase H overexpression in the ΔtopA mutant partially restored the σ³²-dependent induction of stress genes in response to high temperature and ethanol. The presence of overexpressed RNase H also improved the survival rate of the ΔtopA mutant after high temperature and oxidative challenges. Topoisomerase I is likely required during stress response for preventing accumulation of transcription-driven hypernegative supercoiling and R-loop formation at induced stress genes loci.

Original language	English (US)
Pages (from-to)	237-242
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	221
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(03)00209-X
State	Published - Apr 25 2003
Externally published	Yes

Keywords

R-loop
RNase H
Stress response
Top A
Topoisomerase I

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1016/S0378-1097(03)00209-X

Cite this

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title = "RNase H overproduction allows the expression of stress-induced genes in the absence of topoisomerase I",

abstract = "Induction of stress proteins in response to heat shock was found to be reduced significantly in Escherichia coli with ΔtopA mutation. RNase H overexpression in the ΔtopA mutant partially restored the σ32-dependent induction of stress genes in response to high temperature and ethanol. The presence of overexpressed RNase H also improved the survival rate of the ΔtopA mutant after high temperature and oxidative challenges. Topoisomerase I is likely required during stress response for preventing accumulation of transcription-driven hypernegative supercoiling and R-loop formation at induced stress genes loci.",

keywords = "R-loop, RNase H, Stress response, Top A, Topoisomerase I",

author = "Bokun Cheng and Shan Rui and Chengling Ji and Gong, {Vivien W.} and {Van Dyk}, {Tina K.} and Marc Drolet and Tse-Dinh, {Yuk Ching}",

note = "Funding Information: This work was supported by grants GM54226 from NIGMS (to Y.-C.T.-D.) and MT-12667 from the Canadian Institutes of Health Research (to M.D.). ",

year = "2003",

month = apr,

day = "25",

doi = "10.1016/S0378-1097(03)00209-X",

language = "English (US)",

volume = "221",

pages = "237--242",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Wiley-Blackwell",

number = "2",

}

TY - JOUR

T1 - RNase H overproduction allows the expression of stress-induced genes in the absence of topoisomerase I

AU - Cheng, Bokun

AU - Rui, Shan

AU - Ji, Chengling

AU - Gong, Vivien W.

AU - Van Dyk, Tina K.

AU - Drolet, Marc

AU - Tse-Dinh, Yuk Ching

N1 - Funding Information: This work was supported by grants GM54226 from NIGMS (to Y.-C.T.-D.) and MT-12667 from the Canadian Institutes of Health Research (to M.D.).

PY - 2003/4/25

Y1 - 2003/4/25

N2 - Induction of stress proteins in response to heat shock was found to be reduced significantly in Escherichia coli with ΔtopA mutation. RNase H overexpression in the ΔtopA mutant partially restored the σ32-dependent induction of stress genes in response to high temperature and ethanol. The presence of overexpressed RNase H also improved the survival rate of the ΔtopA mutant after high temperature and oxidative challenges. Topoisomerase I is likely required during stress response for preventing accumulation of transcription-driven hypernegative supercoiling and R-loop formation at induced stress genes loci.

AB - Induction of stress proteins in response to heat shock was found to be reduced significantly in Escherichia coli with ΔtopA mutation. RNase H overexpression in the ΔtopA mutant partially restored the σ32-dependent induction of stress genes in response to high temperature and ethanol. The presence of overexpressed RNase H also improved the survival rate of the ΔtopA mutant after high temperature and oxidative challenges. Topoisomerase I is likely required during stress response for preventing accumulation of transcription-driven hypernegative supercoiling and R-loop formation at induced stress genes loci.

KW - R-loop

KW - RNase H

KW - Stress response

KW - Top A

KW - Topoisomerase I

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U2 - 10.1016/S0378-1097(03)00209-X

DO - 10.1016/S0378-1097(03)00209-X

M3 - Article

C2 - 12725933

AN - SCOPUS:0142062412

SN - 0378-1097

VL - 221

SP - 237

EP - 242

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -

RNase H overproduction allows the expression of stress-induced genes in the absence of topoisomerase I

Abstract

Keywords

ASJC Scopus subject areas

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