Ring1B Compacts Chromatin Structure and Represses Gene Expression Independent of Histone Ubiquitination

Ragnhild Eskeland, Martin Leeb, Graeme R. Grimes, Clémence Kress, Shelagh Boyle, Duncan Sproul, Nick Gilbert, Yuhong Fan, Arthur I. Skoultchi, Anton Wutz, Wendy A. Bickmore

Research output: Contribution to journalArticle

326 Citations (Scopus)

Abstract

How polycomb group proteins repress gene expression in vivo is not known. While histone-modifying activities of the polycomb repressive complexes (PRCs) have been studied extensively, in vitro data have suggested a direct activity of the PRC1 complex in compacting chromatin. Here, we investigate higher-order chromatin compaction of polycomb targets in vivo. We show that PRCs are required to maintain a compact chromatin state at Hox loci in embryonic stem cells (ESCs). There is specific decompaction in the absence of PRC2 or PRC1. This is due to a PRC1-like complex, since decompaction occurs in Ring1B null cells that still have PRC2-mediated H3K27 methylation. Moreover, we show that the ability of Ring1B to restore a compact chromatin state and to repress Hox gene expression is not dependent on its histone ubiquitination activity. We suggest that Ring1B-mediated chromatin compaction acts to directly limit transcription in vivo.

Original languageEnglish (US)
Pages (from-to)452-464
Number of pages13
JournalMolecular Cell
Volume38
Issue number3
DOIs
StatePublished - May 14 2010

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Ubiquitination
Histones
Chromatin
Gene Expression
Polycomb-Group Proteins
Null Lymphocytes
Homeobox Genes
Embryonic Stem Cells
Methylation

Keywords

  • DEVBIO
  • DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Eskeland, R., Leeb, M., Grimes, G. R., Kress, C., Boyle, S., Sproul, D., ... Bickmore, W. A. (2010). Ring1B Compacts Chromatin Structure and Represses Gene Expression Independent of Histone Ubiquitination. Molecular Cell, 38(3), 452-464. https://doi.org/10.1016/j.molcel.2010.02.032

Ring1B Compacts Chromatin Structure and Represses Gene Expression Independent of Histone Ubiquitination. / Eskeland, Ragnhild; Leeb, Martin; Grimes, Graeme R.; Kress, Clémence; Boyle, Shelagh; Sproul, Duncan; Gilbert, Nick; Fan, Yuhong; Skoultchi, Arthur I.; Wutz, Anton; Bickmore, Wendy A.

In: Molecular Cell, Vol. 38, No. 3, 14.05.2010, p. 452-464.

Research output: Contribution to journalArticle

Eskeland, R, Leeb, M, Grimes, GR, Kress, C, Boyle, S, Sproul, D, Gilbert, N, Fan, Y, Skoultchi, AI, Wutz, A & Bickmore, WA 2010, 'Ring1B Compacts Chromatin Structure and Represses Gene Expression Independent of Histone Ubiquitination', Molecular Cell, vol. 38, no. 3, pp. 452-464. https://doi.org/10.1016/j.molcel.2010.02.032
Eskeland, Ragnhild ; Leeb, Martin ; Grimes, Graeme R. ; Kress, Clémence ; Boyle, Shelagh ; Sproul, Duncan ; Gilbert, Nick ; Fan, Yuhong ; Skoultchi, Arthur I. ; Wutz, Anton ; Bickmore, Wendy A. / Ring1B Compacts Chromatin Structure and Represses Gene Expression Independent of Histone Ubiquitination. In: Molecular Cell. 2010 ; Vol. 38, No. 3. pp. 452-464.
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