Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment

Olivia Alder, Fabrice Lavial, Anne Helness, Emily Brookes, Sandra I. Pinho, Anil Chandrashekran, Philippe Arnaud, Ana Pombo, Laura O'Neill, Véronique Azuara

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Pluripotent cells develop within the inner cell mass of blastocysts, a mosaic of cells surrounded by an extra-embryonic layer, the trophectoderm. We show that a set of somatic lineage regulators (including Hox, Gata and Sox factors) that carry bivalent chromatin enriched in H3K27me3 and H3K4me2 are selectively targeted by Suv39h1-mediated H3K9me3 and de novo DNA methylation in extra-embryonic versus embryonic (pluripotent) lineages, as assessed both in blastocyst-derived stem cells and in vivo. This stably repressed state is linked with a loss of gene priming for transcription through the exclusion of PRC1 (Ring1B) and RNA polymerase II complexes at bivalent, lineage-inappropriate genes upon trophoblast lineage commitment. Collectively, our results suggest a mutually exclusive role for Ring1B and Suv39h1 in regulating distinct chromatin states at key developmental genes and propose a novel mechanism by which lineage specification can be reinforced during early development.

Original languageEnglish (US)
Pages (from-to)2483-2492
Number of pages10
JournalDevelopment
Volume137
Issue number15
DOIs
StatePublished - Aug 1 2010
Externally publishedYes

Fingerprint

Chromatin
Blastocyst Inner Cell Mass
Developmental Genes
RNA Polymerase II
Trophoblasts
Blastocyst
DNA Methylation
Genes
Stem Cells

Keywords

  • Bivalent chromatin
  • Early development
  • Histone methylation
  • Mouse
  • Silencing
  • Stem cells

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

Alder, O., Lavial, F., Helness, A., Brookes, E., Pinho, S. I., Chandrashekran, A., ... Azuara, V. (2010). Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment. Development, 137(15), 2483-2492. https://doi.org/10.1242/dev.048363

Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment. / Alder, Olivia; Lavial, Fabrice; Helness, Anne; Brookes, Emily; Pinho, Sandra I.; Chandrashekran, Anil; Arnaud, Philippe; Pombo, Ana; O'Neill, Laura; Azuara, Véronique.

In: Development, Vol. 137, No. 15, 01.08.2010, p. 2483-2492.

Research output: Contribution to journalArticle

Alder, O, Lavial, F, Helness, A, Brookes, E, Pinho, SI, Chandrashekran, A, Arnaud, P, Pombo, A, O'Neill, L & Azuara, V 2010, 'Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment', Development, vol. 137, no. 15, pp. 2483-2492. https://doi.org/10.1242/dev.048363
Alder, Olivia ; Lavial, Fabrice ; Helness, Anne ; Brookes, Emily ; Pinho, Sandra I. ; Chandrashekran, Anil ; Arnaud, Philippe ; Pombo, Ana ; O'Neill, Laura ; Azuara, Véronique. / Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment. In: Development. 2010 ; Vol. 137, No. 15. pp. 2483-2492.
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