TY - JOUR
T1 - Ribosomal RNA Sequences of Enterocytozoon bieneusi, Septata intestinalis and Ameson michaelis
T2 - Phylogenetic Construction and Structural Correspondence
AU - ZHU, XIAOLONG
AU - WITTNER, MURRAY
AU - TANOWITZ, HERBERT B.
AU - CALI, ANN
AU - WEISS, LOUIS M.
PY - 1994/5
Y1 - 1994/5
N2 - ABSTRACT. The microsporidian species Enterocytozoon bieneusi, Septata intestinalis and Ameson michaelis were compared by using sequence data of their rRNA gene segments, which were amplified by polymerized chain reaction and directly sequenced. The forward primer 530f (5′‐GTGCCATCCAGCCGCGG‐3′) was in the small subunit rRNA (SSU‐rRNA) and the reverse primer 580r (5′‐GGTCCGTGTTTCAAGACGG‐3′) was in the large subunit rRNA (LSU‐rRNA). We have utilized these sequence data, the published data on Encephalitozoon cuniculi and Encephalitozoon hellem and our cloned SSU‐rRNA genes from E. bieneusi and S. intestinalis to develop a phylogenetic tree for the microsporidia involved in human infection. The higher sequence similarities demonstrated between S. intestinalis and E. cuniculi support the placement of S. intestinalis in the family Encephalitozoonidae. This method of polymerized chain reaction rRNA phylogeny allows the establishment of phylogenetic relationships on limiting material where culture and electron microscopy are difficult or impossible and can be applied to archival material to expand the molecular phylogenetic analysis of the phylum Microspora. In addition, the highly variable region (E. coli numbering 590–650) and intergenic spacer regions in the microsporidia were noted to have structural correspondence, suggesting the possibility that they are coevolving.
AB - ABSTRACT. The microsporidian species Enterocytozoon bieneusi, Septata intestinalis and Ameson michaelis were compared by using sequence data of their rRNA gene segments, which were amplified by polymerized chain reaction and directly sequenced. The forward primer 530f (5′‐GTGCCATCCAGCCGCGG‐3′) was in the small subunit rRNA (SSU‐rRNA) and the reverse primer 580r (5′‐GGTCCGTGTTTCAAGACGG‐3′) was in the large subunit rRNA (LSU‐rRNA). We have utilized these sequence data, the published data on Encephalitozoon cuniculi and Encephalitozoon hellem and our cloned SSU‐rRNA genes from E. bieneusi and S. intestinalis to develop a phylogenetic tree for the microsporidia involved in human infection. The higher sequence similarities demonstrated between S. intestinalis and E. cuniculi support the placement of S. intestinalis in the family Encephalitozoonidae. This method of polymerized chain reaction rRNA phylogeny allows the establishment of phylogenetic relationships on limiting material where culture and electron microscopy are difficult or impossible and can be applied to archival material to expand the molecular phylogenetic analysis of the phylum Microspora. In addition, the highly variable region (E. coli numbering 590–650) and intergenic spacer regions in the microsporidia were noted to have structural correspondence, suggesting the possibility that they are coevolving.
KW - Microsporidia
KW - phylogeny
UR - http://www.scopus.com/inward/record.url?scp=0028426866&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028426866&partnerID=8YFLogxK
U2 - 10.1111/j.1550-7408.1994.tb01498.x
DO - 10.1111/j.1550-7408.1994.tb01498.x
M3 - Article
C2 - 8049683
AN - SCOPUS:0028426866
SN - 1066-5234
VL - 41
SP - 204
EP - 209
JO - Journal of Eukaryotic Microbiology
JF - Journal of Eukaryotic Microbiology
IS - 3
ER -