Ribosomal protein L11 mutations in two functional domains equally affect release factors 1 and 2 activity

Hanae Sato, Koichi Ito, Yoshikazu Nakamura

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Bacterial release factors (RFs) 1 and 2 catalyse translation termination at UAG/UAA and UGA/UAA stop codons respectively. It has been shown that limiting the amount of ribosomal protein L11 affects translation termination at UAG and UGA differently. To understand the functional interplay between L11 and RF1/RF2, we isolated 21 distinct mutations in L11 as suppressors of either temperature-sensitive (ts) RF1/RF2 strains or read-through mutants of lacZ nonsense (UAG or UGA) strains. 10 of 21 mutants restored ts lethal growth of RF1 and/or RF2 strains. All the selected L11 mutants, including the RF1ts- and RF2ts-specific suppressors, had the same effect, either enhancing or reducing, on UAG and UGA termination efficiency in vivo. The specific properties of the selected L11 mutations remained unchanged in an RF3 deletion strain. Moreover, ribosomes absent of L11 had equally reduced activity for both RF1- and RF2-mediated peptide release in vitro. These results suggest that, unlike the previous notion, L11 has a common, cooperative role with RF1 and RF2. These L11 mutations were located on the surface of two domains of L11, and interpreted to affect the interaction between L11 and rRNA or the RFs thereby leading to the altered translation termination.

Original languageEnglish (US)
Pages (from-to)108-120
Number of pages13
JournalMolecular Microbiology
Volume60
Issue number1
DOIs
StatePublished - Apr 2006
Externally publishedYes

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Terminator Codon
Mutation
Temperature
Ribosomes
Peptides
Growth
ribosomal protein L11
In Vitro Techniques

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Ribosomal protein L11 mutations in two functional domains equally affect release factors 1 and 2 activity. / Sato, Hanae; Ito, Koichi; Nakamura, Yoshikazu.

In: Molecular Microbiology, Vol. 60, No. 1, 04.2006, p. 108-120.

Research output: Contribution to journalArticle

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