TY - JOUR
T1 - Ribonuclease III cleavage of bacteriophage T3 RNA polymerase transcripts to late T3 mRNAs
AU - Majumder, H. K.
AU - Bishayee, S.
AU - Chakraborty, P. R.
AU - Maitra, U.
PY - 1977
Y1 - 1977
N2 - In vitro transcription of T3 DNA by T3 phage-induced RNA polymerase (nucleosidetriphosphate : RNA nucleotidyltransferase; EC 2.7.7.6) yields eight discrete RNAs (designated I-VIII) with molecular weights of approximately 6.2, 4.7, 4, 2.8, 1.8, 0.9, 0.52, and 0.21 x 106 respectively. Comparison of the size of in vitro T3 RNA polymerase transcripts with in vivo late T3 mRNAs indicates that several late RNAs produced in T3-infected cells do not correspond to any of the in vitro RNAs and no RNAs as large as the three largest in vitro RNA species, I, II, and III are observed. Escherichia coli RNase III cleaves these three high molecular weight T3 RNA polymerase transcripts to discrete RNAs that comigrate in polyacrylamide gel electrophoresis with some of the late T3 RNAs.
AB - In vitro transcription of T3 DNA by T3 phage-induced RNA polymerase (nucleosidetriphosphate : RNA nucleotidyltransferase; EC 2.7.7.6) yields eight discrete RNAs (designated I-VIII) with molecular weights of approximately 6.2, 4.7, 4, 2.8, 1.8, 0.9, 0.52, and 0.21 x 106 respectively. Comparison of the size of in vitro T3 RNA polymerase transcripts with in vivo late T3 mRNAs indicates that several late RNAs produced in T3-infected cells do not correspond to any of the in vitro RNAs and no RNAs as large as the three largest in vitro RNA species, I, II, and III are observed. Escherichia coli RNase III cleaves these three high molecular weight T3 RNA polymerase transcripts to discrete RNAs that comigrate in polyacrylamide gel electrophoresis with some of the late T3 RNAs.
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U2 - 10.1073/pnas.74.11.4891
DO - 10.1073/pnas.74.11.4891
M3 - Article
C2 - 337303
AN - SCOPUS:0017666165
SN - 0027-8424
VL - 74
SP - 4891
EP - 4894
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 11
ER -