RhoA/ROCK-mediated switching between Cdc42- and Rac1-dependent protrusion in MTLn3 carcinoma cells

Mirvat El-Sibai, Olivier Pertz, Huan Pang, Shu Chin Yip, Mike Lorenz, Marc Symons, John S. Condeelis, Klaus M. Hahn, Jonathan M. Backer

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

Rho GTPases are versatile regulators of cell shape that act on the actin cytoskeleton. Studies using Rho GTPase mutants have shown that, in some cells, Rac1 and Cdc42 regulate the formation of lamellipodia and filopodia, respectively at the leading edge, whereas RhoA mediates contraction at the rear of moving cells. However, recent reports have described a zone of RhoA/ROCK activation at the front of cells undergoing motility. In this study, we use a FRET-based RhoA biosensor to show that RhoA activation localizes to the leading edge of EGF-stimulated cells. Inhibition of Rho or ROCK enhanced protrusion, yet markedly inhibited cell motility; these changes correlated with a marked activation of Rac-1 at the cell edge. Surprisingly, whereas EGF-stimulated protrusion in control MTLn3 cells is Rac-independent and Cdc42-dependent, the opposite pattern is observed in MTLn3 cells after inhibition of ROCK. Thus, Rho and ROCK suppress Rac-1 activation at the leading edge, and inhibition of ROCK causes a switch between Cdc42 and Rac-1 as the dominant Rho GTPase driving protrusion in carcinoma cells. These data describe a novel role for Rho in coordinating signaling by Rac and Cdc42.

Original languageEnglish (US)
Pages (from-to)1540-1552
Number of pages13
JournalExperimental Cell Research
Volume314
Issue number7
DOIs
StatePublished - Apr 15 2008

Keywords

  • EGF
  • Metastasis
  • ROCK
  • Rac
  • RhoA

ASJC Scopus subject areas

  • Cell Biology

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