Rho GTPase isoforms in cell motility

Don't fret, we have FRET

Sara K. Donnelly, Jose Javier Bravo-Cordero, Louis Hodgson

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The Rho-family of p21 small GTPases are directly linked to the regulation of actin-based motile machinery and play a key role in the control of cell migration. Aside from the original and most well-characterized canonical Rho GTPases RhoA, Rac1, and Cdc42, numerous isoforms of these key proteins have been identified and shown to have specific roles in regulating various cellular motility processes. The major difficulty in addressing these isoform-specific effects is that isoforms typically contain highly similar primary amino acid sequences and thus are able to interact with the same upstream regulators and the downstream effector targets. Here, we will introduce the major members of each GTPase subfamily and discuss recent advances in the design and application of fluorescent resonance energy transfer-based probes, which are at the forefront of the technologies available to directly probe the differential, spatiotemporal activation dynamics of these proteins in live single cells. Currently, it is possible to specifically detect the activation status of RhoA vs. RhoC isoforms, as well as Cdc42 vs. TC-10 isoforms in living cells. Clearly, additional efforts are still required to produce biosensor systems capable of detecting other isoforms of Rho GTPases including RhoB, Rac2/3, RhoG, etc. Through such efforts, we will uncover the isoform-specific roles of these near-identical proteins in living cells, clearly an important area of the Rho GTPase biology that is not yet fully appreciated.

Original languageEnglish (US)
Pages (from-to)526-534
Number of pages9
JournalCell Adhesion and Migration
Volume8
Issue number6
DOIs
StatePublished - Nov 1 2014

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rho GTP-Binding Proteins
Cell Movement
Protein Isoforms
Monomeric GTP-Binding Proteins
GTP Phosphohydrolases
Energy Transfer
Biosensing Techniques
Actins
Amino Acid Sequence
Proteins
Technology

ASJC Scopus subject areas

  • Cell Biology
  • Cellular and Molecular Neuroscience

Cite this

Rho GTPase isoforms in cell motility : Don't fret, we have FRET. / Donnelly, Sara K.; Bravo-Cordero, Jose Javier; Hodgson, Louis.

In: Cell Adhesion and Migration, Vol. 8, No. 6, 01.11.2014, p. 526-534.

Research output: Contribution to journalArticle

Donnelly, Sara K. ; Bravo-Cordero, Jose Javier ; Hodgson, Louis. / Rho GTPase isoforms in cell motility : Don't fret, we have FRET. In: Cell Adhesion and Migration. 2014 ; Vol. 8, No. 6. pp. 526-534.
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