Rheology of hemoglobin S gels: Possible correlation with impaired microvascular circulation

Robin W. Briehl

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The sequence of pathogenic events in sickle cell disease begins with the genetic abnormality and proceeds through molecular and red cell abnormalities to clinical events of vascular obstruction, hemolysis, and crisis. The least studied event, central in the sequence, is altered viscosity and rheology of the gelled deoxyhemoglobin S. In this work, shear is shown not only to measure the formation of gels, but to alter the progress of gelation. Thus, intraerythrocytic shear may be an important factor in pathogenesis. Increasing shear decreases the delay time for gelation as measured directly and by experiments in which shear rate is altered during the delay period. After the delay time, during the growth stage, characterized by a large increase in viscosity, shearing increases the rate of viscosity increase. On the other hand, as previously shown, shearing breaks down solid-like gels. These two effects of shear, one detrimental and the other possibly beneficial, may contribute to the variations known to exist in the clinical picture of sickle cell disease. The growth stage progress curve of gelation is here shown to he exponential in shape. This suggests that fiber breakage occurs under shear and/or that new fibers nucleate on the surface of existing fibers (i.e. heterogeneous nucleation). Finally, the progress curve is shown to be composed of plastic (i.e. solid-like) as well as viscous components early in gel development.

Original languageEnglish (US)
Pages (from-to)390-398
Number of pages9
JournalJournal of Pediatric Hematology/Oncology
Volume5
Issue number4
StatePublished - 1983

Fingerprint

Sickle Hemoglobin
Rheology
Viscosity
Gels
Sickle Cell Anemia
Growth
Hemolysis
Plastics
Blood Vessels

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Pediatrics, Perinatology, and Child Health

Cite this

Rheology of hemoglobin S gels : Possible correlation with impaired microvascular circulation. / Briehl, Robin W.

In: Journal of Pediatric Hematology/Oncology, Vol. 5, No. 4, 1983, p. 390-398.

Research output: Contribution to journalArticle

@article{f80b18d111a74298a1adc5c00b4e179d,
title = "Rheology of hemoglobin S gels: Possible correlation with impaired microvascular circulation",
abstract = "The sequence of pathogenic events in sickle cell disease begins with the genetic abnormality and proceeds through molecular and red cell abnormalities to clinical events of vascular obstruction, hemolysis, and crisis. The least studied event, central in the sequence, is altered viscosity and rheology of the gelled deoxyhemoglobin S. In this work, shear is shown not only to measure the formation of gels, but to alter the progress of gelation. Thus, intraerythrocytic shear may be an important factor in pathogenesis. Increasing shear decreases the delay time for gelation as measured directly and by experiments in which shear rate is altered during the delay period. After the delay time, during the growth stage, characterized by a large increase in viscosity, shearing increases the rate of viscosity increase. On the other hand, as previously shown, shearing breaks down solid-like gels. These two effects of shear, one detrimental and the other possibly beneficial, may contribute to the variations known to exist in the clinical picture of sickle cell disease. The growth stage progress curve of gelation is here shown to he exponential in shape. This suggests that fiber breakage occurs under shear and/or that new fibers nucleate on the surface of existing fibers (i.e. heterogeneous nucleation). Finally, the progress curve is shown to be composed of plastic (i.e. solid-like) as well as viscous components early in gel development.",
author = "Briehl, {Robin W.}",
year = "1983",
language = "English (US)",
volume = "5",
pages = "390--398",
journal = "Journal of Pediatric Hematology/Oncology",
issn = "1077-4114",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Rheology of hemoglobin S gels

T2 - Possible correlation with impaired microvascular circulation

AU - Briehl, Robin W.

PY - 1983

Y1 - 1983

N2 - The sequence of pathogenic events in sickle cell disease begins with the genetic abnormality and proceeds through molecular and red cell abnormalities to clinical events of vascular obstruction, hemolysis, and crisis. The least studied event, central in the sequence, is altered viscosity and rheology of the gelled deoxyhemoglobin S. In this work, shear is shown not only to measure the formation of gels, but to alter the progress of gelation. Thus, intraerythrocytic shear may be an important factor in pathogenesis. Increasing shear decreases the delay time for gelation as measured directly and by experiments in which shear rate is altered during the delay period. After the delay time, during the growth stage, characterized by a large increase in viscosity, shearing increases the rate of viscosity increase. On the other hand, as previously shown, shearing breaks down solid-like gels. These two effects of shear, one detrimental and the other possibly beneficial, may contribute to the variations known to exist in the clinical picture of sickle cell disease. The growth stage progress curve of gelation is here shown to he exponential in shape. This suggests that fiber breakage occurs under shear and/or that new fibers nucleate on the surface of existing fibers (i.e. heterogeneous nucleation). Finally, the progress curve is shown to be composed of plastic (i.e. solid-like) as well as viscous components early in gel development.

AB - The sequence of pathogenic events in sickle cell disease begins with the genetic abnormality and proceeds through molecular and red cell abnormalities to clinical events of vascular obstruction, hemolysis, and crisis. The least studied event, central in the sequence, is altered viscosity and rheology of the gelled deoxyhemoglobin S. In this work, shear is shown not only to measure the formation of gels, but to alter the progress of gelation. Thus, intraerythrocytic shear may be an important factor in pathogenesis. Increasing shear decreases the delay time for gelation as measured directly and by experiments in which shear rate is altered during the delay period. After the delay time, during the growth stage, characterized by a large increase in viscosity, shearing increases the rate of viscosity increase. On the other hand, as previously shown, shearing breaks down solid-like gels. These two effects of shear, one detrimental and the other possibly beneficial, may contribute to the variations known to exist in the clinical picture of sickle cell disease. The growth stage progress curve of gelation is here shown to he exponential in shape. This suggests that fiber breakage occurs under shear and/or that new fibers nucleate on the surface of existing fibers (i.e. heterogeneous nucleation). Finally, the progress curve is shown to be composed of plastic (i.e. solid-like) as well as viscous components early in gel development.

UR - http://www.scopus.com/inward/record.url?scp=0021044672&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021044672&partnerID=8YFLogxK

M3 - Article

C2 - 6670719

AN - SCOPUS:0021044672

VL - 5

SP - 390

EP - 398

JO - Journal of Pediatric Hematology/Oncology

JF - Journal of Pediatric Hematology/Oncology

SN - 1077-4114

IS - 4

ER -