Revisiting a protocol for extraction of mycobacterial lipids

Pooja Singh, Rajesh Sinha, Rashmi Tandon, Gaurav Tyagi, Preeti Khatri, L. Chandra Shekhar Reddy, Neeraj K. Saini, Rakesh Pathak, Mandira Varma-Basil, Ashok K. Prasad, Mridula Bose

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Determination of lipid content of any biological sample is essential for various kinds of studies related to pathogenicity and drug development. Thus, reliable methods for the quantitative extraction of lipids are of critical importance. The mycobacterial cell wall is largely composed of lipids. Commonly used methods to extract lipids, such as the Bligh and Dyer method or the Folch method, yield a low amount of lipids when applied to mycobacterial cells. This study presents an efficient modification of Chandramauli's method, a less known method developed at this institute earlier that is able to yield a considerably higher concentration of mycobacterial lipids.

Original languageEnglish (US)
Pages (from-to)168-172
Number of pages5
JournalInternational Journal of Mycobacteriology
Volume3
Issue number3
DOIs
StatePublished - Sep 1 2014
Externally publishedYes

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Lipids
Cell Wall
Virulence
Pharmaceutical Preparations

Keywords

  • M. tuberculosis
  • Thin layer chromatography
  • Total lipids

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology (medical)

Cite this

Singh, P., Sinha, R., Tandon, R., Tyagi, G., Khatri, P., Chandra Shekhar Reddy, L., ... Bose, M. (2014). Revisiting a protocol for extraction of mycobacterial lipids. International Journal of Mycobacteriology, 3(3), 168-172. https://doi.org/10.1016/j.ijmyco.2014.07.008

Revisiting a protocol for extraction of mycobacterial lipids. / Singh, Pooja; Sinha, Rajesh; Tandon, Rashmi; Tyagi, Gaurav; Khatri, Preeti; Chandra Shekhar Reddy, L.; Saini, Neeraj K.; Pathak, Rakesh; Varma-Basil, Mandira; Prasad, Ashok K.; Bose, Mridula.

In: International Journal of Mycobacteriology, Vol. 3, No. 3, 01.09.2014, p. 168-172.

Research output: Contribution to journalArticle

Singh, P, Sinha, R, Tandon, R, Tyagi, G, Khatri, P, Chandra Shekhar Reddy, L, Saini, NK, Pathak, R, Varma-Basil, M, Prasad, AK & Bose, M 2014, 'Revisiting a protocol for extraction of mycobacterial lipids', International Journal of Mycobacteriology, vol. 3, no. 3, pp. 168-172. https://doi.org/10.1016/j.ijmyco.2014.07.008
Singh P, Sinha R, Tandon R, Tyagi G, Khatri P, Chandra Shekhar Reddy L et al. Revisiting a protocol for extraction of mycobacterial lipids. International Journal of Mycobacteriology. 2014 Sep 1;3(3):168-172. https://doi.org/10.1016/j.ijmyco.2014.07.008
Singh, Pooja ; Sinha, Rajesh ; Tandon, Rashmi ; Tyagi, Gaurav ; Khatri, Preeti ; Chandra Shekhar Reddy, L. ; Saini, Neeraj K. ; Pathak, Rakesh ; Varma-Basil, Mandira ; Prasad, Ashok K. ; Bose, Mridula. / Revisiting a protocol for extraction of mycobacterial lipids. In: International Journal of Mycobacteriology. 2014 ; Vol. 3, No. 3. pp. 168-172.
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