Abstract
Determination of lipid content of any biological sample is essential for various kinds of studies related to pathogenicity and drug development. Thus, reliable methods for the quantitative extraction of lipids are of critical importance. The mycobacterial cell wall is largely composed of lipids. Commonly used methods to extract lipids, such as the Bligh and Dyer method or the Folch method, yield a low amount of lipids when applied to mycobacterial cells. This study presents an efficient modification of Chandramauli's method, a less known method developed at this institute earlier that is able to yield a considerably higher concentration of mycobacterial lipids.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 168-172 |
| Number of pages | 5 |
| Journal | International Journal of Mycobacteriology |
| Volume | 3 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 1 2014 |
| Externally published | Yes |
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Keywords
- M. tuberculosis
- Thin layer chromatography
- Total lipids
ASJC Scopus subject areas
- Infectious Diseases
- Microbiology (medical)
Cite this
Revisiting a protocol for extraction of mycobacterial lipids. / Singh, Pooja; Sinha, Rajesh; Tandon, Rashmi; Tyagi, Gaurav; Khatri, Preeti; Chandra Shekhar Reddy, L.; Saini, Neeraj K.; Pathak, Rakesh; Varma-Basil, Mandira; Prasad, Ashok K.; Bose, Mridula.
In: International Journal of Mycobacteriology, Vol. 3, No. 3, 01.09.2014, p. 168-172.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Revisiting a protocol for extraction of mycobacterial lipids
AU - Singh, Pooja
AU - Sinha, Rajesh
AU - Tandon, Rashmi
AU - Tyagi, Gaurav
AU - Khatri, Preeti
AU - Chandra Shekhar Reddy, L.
AU - Saini, Neeraj K.
AU - Pathak, Rakesh
AU - Varma-Basil, Mandira
AU - Prasad, Ashok K.
AU - Bose, Mridula
PY - 2014/9/1
Y1 - 2014/9/1
N2 - Determination of lipid content of any biological sample is essential for various kinds of studies related to pathogenicity and drug development. Thus, reliable methods for the quantitative extraction of lipids are of critical importance. The mycobacterial cell wall is largely composed of lipids. Commonly used methods to extract lipids, such as the Bligh and Dyer method or the Folch method, yield a low amount of lipids when applied to mycobacterial cells. This study presents an efficient modification of Chandramauli's method, a less known method developed at this institute earlier that is able to yield a considerably higher concentration of mycobacterial lipids.
AB - Determination of lipid content of any biological sample is essential for various kinds of studies related to pathogenicity and drug development. Thus, reliable methods for the quantitative extraction of lipids are of critical importance. The mycobacterial cell wall is largely composed of lipids. Commonly used methods to extract lipids, such as the Bligh and Dyer method or the Folch method, yield a low amount of lipids when applied to mycobacterial cells. This study presents an efficient modification of Chandramauli's method, a less known method developed at this institute earlier that is able to yield a considerably higher concentration of mycobacterial lipids.
KW - M. tuberculosis
KW - Thin layer chromatography
KW - Total lipids
UR - http://www.scopus.com/inward/record.url?scp=84908010435&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84908010435&partnerID=8YFLogxK
U2 - 10.1016/j.ijmyco.2014.07.008
DO - 10.1016/j.ijmyco.2014.07.008
M3 - Article
AN - SCOPUS:84908010435
VL - 3
SP - 168
EP - 172
JO - International Journal of Mycobacteriology
JF - International Journal of Mycobacteriology
SN - 2212-5531
IS - 3
ER -