Revisiting a protocol for extraction of mycobacterial lipids

Pooja Singh; Rajesh Sinha; Rashmi Tandon; Gaurav Tyagi; Preeti Khatri; L. Chandra Shekhar Reddy; Neeraj K. Saini; Rakesh Pathak; Mandira Varma-Basil; Ashok K. Prasad; Mridula Bose

doi:10.1016/j.ijmyco.2014.07.008

Revisiting a protocol for extraction of mycobacterial lipids

Pooja Singh, Rajesh Sinha, Rashmi Tandon, Gaurav Tyagi, Preeti Khatri, L. Chandra Shekhar Reddy, Neeraj K. Saini, Rakesh Pathak, Mandira Varma-Basil, Ashok K. Prasad, Mridula Bose

Microbiology & Immunology

Research output: Contribution to journal › Article

4 Scopus citations

Abstract

Determination of lipid content of any biological sample is essential for various kinds of studies related to pathogenicity and drug development. Thus, reliable methods for the quantitative extraction of lipids are of critical importance. The mycobacterial cell wall is largely composed of lipids. Commonly used methods to extract lipids, such as the Bligh and Dyer method or the Folch method, yield a low amount of lipids when applied to mycobacterial cells. This study presents an efficient modification of Chandramauli's method, a less known method developed at this institute earlier that is able to yield a considerably higher concentration of mycobacterial lipids.

Original language	English (US)
Pages (from-to)	168-172
Number of pages	5
Journal	International Journal of Mycobacteriology
Volume	3
Issue number	3
DOIs	https://doi.org/10.1016/j.ijmyco.2014.07.008
Publication status	Published - Sep 1 2014

Fingerprint

Keywords

M. tuberculosis
Thin layer chromatography
Total lipids

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

Cite this

Singh, P., Sinha, R., Tandon, R., Tyagi, G., Khatri, P., Chandra Shekhar Reddy, L., ... Bose, M. (2014). Revisiting a protocol for extraction of mycobacterial lipids. International Journal of Mycobacteriology, 3(3), 168-172. https://doi.org/10.1016/j.ijmyco.2014.07.008

Revisiting a protocol for extraction of mycobacterial lipids. / Singh, Pooja; Sinha, Rajesh; Tandon, Rashmi; Tyagi, Gaurav; Khatri, Preeti; Chandra Shekhar Reddy, L.; Saini, Neeraj K.; Pathak, Rakesh; Varma-Basil, Mandira; Prasad, Ashok K.; Bose, Mridula.

In: International Journal of Mycobacteriology, Vol. 3, No. 3, 01.09.2014, p. 168-172.

Research output: Contribution to journal › Article

@article{108fd05e67c9403a902001011ffa549a,

title = "Revisiting a protocol for extraction of mycobacterial lipids",

abstract = "Determination of lipid content of any biological sample is essential for various kinds of studies related to pathogenicity and drug development. Thus, reliable methods for the quantitative extraction of lipids are of critical importance. The mycobacterial cell wall is largely composed of lipids. Commonly used methods to extract lipids, such as the Bligh and Dyer method or the Folch method, yield a low amount of lipids when applied to mycobacterial cells. This study presents an efficient modification of Chandramauli's method, a less known method developed at this institute earlier that is able to yield a considerably higher concentration of mycobacterial lipids.",

keywords = "M. tuberculosis, Thin layer chromatography, Total lipids",

author = "Pooja Singh and Rajesh Sinha and Rashmi Tandon and Gaurav Tyagi and Preeti Khatri and {Chandra Shekhar Reddy}, L. and Saini, {Neeraj K.} and Rakesh Pathak and Mandira Varma-Basil and Prasad, {Ashok K.} and Mridula Bose",

year = "2014",

month = "9",

day = "1",

doi = "10.1016/j.ijmyco.2014.07.008",

language = "English (US)",

volume = "3",

pages = "168--172",

journal = "International Journal of Mycobacteriology",

issn = "2212-5531",

publisher = "Elsevier Inc.",

number = "3",

}

TY - JOUR

T1 - Revisiting a protocol for extraction of mycobacterial lipids

AU - Singh, Pooja

AU - Sinha, Rajesh

AU - Tandon, Rashmi

AU - Tyagi, Gaurav

AU - Khatri, Preeti

AU - Chandra Shekhar Reddy, L.

AU - Saini, Neeraj K.

AU - Pathak, Rakesh

AU - Varma-Basil, Mandira

AU - Prasad, Ashok K.

AU - Bose, Mridula

PY - 2014/9/1

Y1 - 2014/9/1

N2 - Determination of lipid content of any biological sample is essential for various kinds of studies related to pathogenicity and drug development. Thus, reliable methods for the quantitative extraction of lipids are of critical importance. The mycobacterial cell wall is largely composed of lipids. Commonly used methods to extract lipids, such as the Bligh and Dyer method or the Folch method, yield a low amount of lipids when applied to mycobacterial cells. This study presents an efficient modification of Chandramauli's method, a less known method developed at this institute earlier that is able to yield a considerably higher concentration of mycobacterial lipids.

AB - Determination of lipid content of any biological sample is essential for various kinds of studies related to pathogenicity and drug development. Thus, reliable methods for the quantitative extraction of lipids are of critical importance. The mycobacterial cell wall is largely composed of lipids. Commonly used methods to extract lipids, such as the Bligh and Dyer method or the Folch method, yield a low amount of lipids when applied to mycobacterial cells. This study presents an efficient modification of Chandramauli's method, a less known method developed at this institute earlier that is able to yield a considerably higher concentration of mycobacterial lipids.

KW - M. tuberculosis

KW - Thin layer chromatography

KW - Total lipids

UR - http://www.scopus.com/inward/record.url?scp=84908010435&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908010435&partnerID=8YFLogxK

U2 - 10.1016/j.ijmyco.2014.07.008

DO - 10.1016/j.ijmyco.2014.07.008

M3 - Article

AN - SCOPUS:84908010435

VL - 3

SP - 168

EP - 172

JO - International Journal of Mycobacteriology

JF - International Journal of Mycobacteriology

SN - 2212-5531

IS - 3

ER -

Access to Document

10.1016/j.ijmyco.2014.07.008