Reversibly switchable photoacoustic tomography using a genetically encoded near-infrared phytochrome

Junjie Yao, Andrii Kaberniuk, Lei Li, Daria Shcherbakova, Ruiying Zhang, Lidai Wang, Guo Li, Vladislav Verkhusha, Lihong V. Wanga

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Optical imaging of genetically encoded probes has revolutionized biomedical studies by providing valuable information about targeted biological processes. Here, we report a novel imaging technique, termed reversibly switchable photoacoustic tomography (RS-PAT), which exhibits large penetration depth, high detection sensitivity, and super-resolution. RS-PAT combines advanced photoacoustic imaging techniques with, for the first time, a nonfluorescent photoswitchable bacterial phytochrome. This bacterial phytochrome is the most near-infrared shifted genetically encoded probe reported so far. Moreover, this bacterial phytochrome is reversibly photoconvertible between its far-red and near-infrared light absorption states. Taking maximum advantage of the powerful imaging capability of PAT and the unique photochemical properties of the phytochrome, RS-PAT has broken through both the optical diffusion limit for deep-tissue imaging and the optical diffraction limit for super-resolution photoacoustic microscopy. Specifically, with RS-PAT we have achieved an unprecedented detection sensitivity of ∼2 μM, or as few as ∼20 tumor cells, at a centimeter depth. Such high sensitivity is fully demonstrated in our study by monitoring tumor growth and metastasis at whole-body level with ∼100 μm resolution. Moreover, our microscopic implementation of RS-PAT is capable of imaging mammalian cells with a sub-diffraction lateral resolution of ∼140 nm and axial resolution of ∼400 nm, which are respectively ∼2-fold and ∼75-fold finer than those of our conventional photoacoustic microscopy. Overall, RS-PAT is a new and promising imaging technology for studying biological processes at different length scales.

Original languageEnglish (US)
Title of host publicationPhotons Plus Ultrasound: Imaging and Sensing 2016
PublisherSPIE
Volume9708
ISBN (Electronic)9781628419429
DOIs
StatePublished - 2016
EventPhotons Plus Ultrasound: Imaging and Sensing 2016 - San Francisco, United States
Duration: Feb 14 2016Feb 17 2016

Other

OtherPhotons Plus Ultrasound: Imaging and Sensing 2016
CountryUnited States
CitySan Francisco
Period2/14/162/17/16

Fingerprint

phytochrome
Phytochrome
Photoacoustic effect
Tomography
tomography
Infrared radiation
Imaging techniques
photoacoustic microscopy
Photoacoustic microscopy
Biological Phenomena
Optical Imaging
imaging techniques
Photoacoustic Techniques
Microscopy
tumors
Tumors
Diffraction
Cells
probes
metastasis

Keywords

  • Bacterial phytochrome
  • Deep tissue imaging
  • Genetic imaging
  • Near-infrared protein
  • Photoacoustic microscopy
  • Photoacoustic tomography
  • Reversibly switchable protein
  • Super-resolution imaging

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Yao, J., Kaberniuk, A., Li, L., Shcherbakova, D., Zhang, R., Wang, L., ... Wanga, L. V. (2016). Reversibly switchable photoacoustic tomography using a genetically encoded near-infrared phytochrome. In Photons Plus Ultrasound: Imaging and Sensing 2016 (Vol. 9708). [97082U] SPIE. https://doi.org/10.1117/12.2229156

Reversibly switchable photoacoustic tomography using a genetically encoded near-infrared phytochrome. / Yao, Junjie; Kaberniuk, Andrii; Li, Lei; Shcherbakova, Daria; Zhang, Ruiying; Wang, Lidai; Li, Guo; Verkhusha, Vladislav; Wanga, Lihong V.

Photons Plus Ultrasound: Imaging and Sensing 2016. Vol. 9708 SPIE, 2016. 97082U.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yao, J, Kaberniuk, A, Li, L, Shcherbakova, D, Zhang, R, Wang, L, Li, G, Verkhusha, V & Wanga, LV 2016, Reversibly switchable photoacoustic tomography using a genetically encoded near-infrared phytochrome. in Photons Plus Ultrasound: Imaging and Sensing 2016. vol. 9708, 97082U, SPIE, Photons Plus Ultrasound: Imaging and Sensing 2016, San Francisco, United States, 2/14/16. https://doi.org/10.1117/12.2229156
Yao J, Kaberniuk A, Li L, Shcherbakova D, Zhang R, Wang L et al. Reversibly switchable photoacoustic tomography using a genetically encoded near-infrared phytochrome. In Photons Plus Ultrasound: Imaging and Sensing 2016. Vol. 9708. SPIE. 2016. 97082U https://doi.org/10.1117/12.2229156
Yao, Junjie ; Kaberniuk, Andrii ; Li, Lei ; Shcherbakova, Daria ; Zhang, Ruiying ; Wang, Lidai ; Li, Guo ; Verkhusha, Vladislav ; Wanga, Lihong V. / Reversibly switchable photoacoustic tomography using a genetically encoded near-infrared phytochrome. Photons Plus Ultrasound: Imaging and Sensing 2016. Vol. 9708 SPIE, 2016.
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