Abstract
Optical imaging of genetically encoded probes has revolutionized biomedical studies by providing valuable information about targeted biological processes. Here, we report a novel imaging technique, termed reversibly switchable photoacoustic tomography (RS-PAT), which exhibits large penetration depth, high detection sensitivity, and super-resolution. RS-PAT combines advanced photoacoustic imaging techniques with, for the first time, a nonfluorescent photoswitchable bacterial phytochrome. This bacterial phytochrome is the most near-infrared shifted genetically encoded probe reported so far. Moreover, this bacterial phytochrome is reversibly photoconvertible between its far-red and near-infrared light absorption states. Taking maximum advantage of the powerful imaging capability of PAT and the unique photochemical properties of the phytochrome, RS-PAT has broken through both the optical diffusion limit for deep-tissue imaging and the optical diffraction limit for super-resolution photoacoustic microscopy. Specifically, with RS-PAT we have achieved an unprecedented detection sensitivity of ∼2 μM, or as few as ∼20 tumor cells, at a centimeter depth. Such high sensitivity is fully demonstrated in our study by monitoring tumor growth and metastasis at whole-body level with ∼100 μm resolution. Moreover, our microscopic implementation of RS-PAT is capable of imaging mammalian cells with a sub-diffraction lateral resolution of ∼140 nm and axial resolution of ∼400 nm, which are respectively ∼2-fold and ∼75-fold finer than those of our conventional photoacoustic microscopy. Overall, RS-PAT is a new and promising imaging technology for studying biological processes at different length scales.
| Original language | English (US) |
|---|---|
| Title of host publication | Photons Plus Ultrasound: Imaging and Sensing 2016 |
| Publisher | SPIE |
| Volume | 9708 |
| ISBN (Electronic) | 9781628419429 |
| DOIs | |
| State | Published - 2016 |
| Event | Photons Plus Ultrasound: Imaging and Sensing 2016 - San Francisco, United States Duration: Feb 14 2016 → Feb 17 2016 |
Other
| Other | Photons Plus Ultrasound: Imaging and Sensing 2016 |
|---|---|
| Country | United States |
| City | San Francisco |
| Period | 2/14/16 → 2/17/16 |
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Keywords
- Bacterial phytochrome
- Deep tissue imaging
- Genetic imaging
- Near-infrared protein
- Photoacoustic microscopy
- Photoacoustic tomography
- Reversibly switchable protein
- Super-resolution imaging
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Radiology Nuclear Medicine and imaging
Cite this
Reversibly switchable photoacoustic tomography using a genetically encoded near-infrared phytochrome. / Yao, Junjie; Kaberniuk, Andrii; Li, Lei; Shcherbakova, Daria; Zhang, Ruiying; Wang, Lidai; Li, Guo; Verkhusha, Vladislav; Wanga, Lihong V.
Photons Plus Ultrasound: Imaging and Sensing 2016. Vol. 9708 SPIE, 2016. 97082U.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Reversibly switchable photoacoustic tomography using a genetically encoded near-infrared phytochrome
AU - Yao, Junjie
AU - Kaberniuk, Andrii
AU - Li, Lei
AU - Shcherbakova, Daria
AU - Zhang, Ruiying
AU - Wang, Lidai
AU - Li, Guo
AU - Verkhusha, Vladislav
AU - Wanga, Lihong V.
PY - 2016
Y1 - 2016
N2 - Optical imaging of genetically encoded probes has revolutionized biomedical studies by providing valuable information about targeted biological processes. Here, we report a novel imaging technique, termed reversibly switchable photoacoustic tomography (RS-PAT), which exhibits large penetration depth, high detection sensitivity, and super-resolution. RS-PAT combines advanced photoacoustic imaging techniques with, for the first time, a nonfluorescent photoswitchable bacterial phytochrome. This bacterial phytochrome is the most near-infrared shifted genetically encoded probe reported so far. Moreover, this bacterial phytochrome is reversibly photoconvertible between its far-red and near-infrared light absorption states. Taking maximum advantage of the powerful imaging capability of PAT and the unique photochemical properties of the phytochrome, RS-PAT has broken through both the optical diffusion limit for deep-tissue imaging and the optical diffraction limit for super-resolution photoacoustic microscopy. Specifically, with RS-PAT we have achieved an unprecedented detection sensitivity of ∼2 μM, or as few as ∼20 tumor cells, at a centimeter depth. Such high sensitivity is fully demonstrated in our study by monitoring tumor growth and metastasis at whole-body level with ∼100 μm resolution. Moreover, our microscopic implementation of RS-PAT is capable of imaging mammalian cells with a sub-diffraction lateral resolution of ∼140 nm and axial resolution of ∼400 nm, which are respectively ∼2-fold and ∼75-fold finer than those of our conventional photoacoustic microscopy. Overall, RS-PAT is a new and promising imaging technology for studying biological processes at different length scales.
AB - Optical imaging of genetically encoded probes has revolutionized biomedical studies by providing valuable information about targeted biological processes. Here, we report a novel imaging technique, termed reversibly switchable photoacoustic tomography (RS-PAT), which exhibits large penetration depth, high detection sensitivity, and super-resolution. RS-PAT combines advanced photoacoustic imaging techniques with, for the first time, a nonfluorescent photoswitchable bacterial phytochrome. This bacterial phytochrome is the most near-infrared shifted genetically encoded probe reported so far. Moreover, this bacterial phytochrome is reversibly photoconvertible between its far-red and near-infrared light absorption states. Taking maximum advantage of the powerful imaging capability of PAT and the unique photochemical properties of the phytochrome, RS-PAT has broken through both the optical diffusion limit for deep-tissue imaging and the optical diffraction limit for super-resolution photoacoustic microscopy. Specifically, with RS-PAT we have achieved an unprecedented detection sensitivity of ∼2 μM, or as few as ∼20 tumor cells, at a centimeter depth. Such high sensitivity is fully demonstrated in our study by monitoring tumor growth and metastasis at whole-body level with ∼100 μm resolution. Moreover, our microscopic implementation of RS-PAT is capable of imaging mammalian cells with a sub-diffraction lateral resolution of ∼140 nm and axial resolution of ∼400 nm, which are respectively ∼2-fold and ∼75-fold finer than those of our conventional photoacoustic microscopy. Overall, RS-PAT is a new and promising imaging technology for studying biological processes at different length scales.
KW - Bacterial phytochrome
KW - Deep tissue imaging
KW - Genetic imaging
KW - Near-infrared protein
KW - Photoacoustic microscopy
KW - Photoacoustic tomography
KW - Reversibly switchable protein
KW - Super-resolution imaging
UR - http://www.scopus.com/inward/record.url?scp=84975041695&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84975041695&partnerID=8YFLogxK
U2 - 10.1117/12.2229156
DO - 10.1117/12.2229156
M3 - Conference contribution
AN - SCOPUS:84975041695
VL - 9708
BT - Photons Plus Ultrasound: Imaging and Sensing 2016
PB - SPIE
ER -