Retroviral restriction factor TRIM5α is a trimer

Claudia C. Mische, Hassan Javanbakht, Byeongwoon Song, Felipe Diaz-Griffero, Matthew Stremlau, Bettina Strack, Zhihai Si, Joseph Sodroski

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

The retrovirus restriction factor TRIM5αa targets the viral capsid soon after entry. Here we show that the TRIM5α protein oligomerizes into trimers. The TRIM5α coiled-coil and B30.2(SPRY) domains make important contributions to the formation and/or stability of the trimers. A functionally defective TRIM5α mutant with the RING and B-box 2 domains deleted can form heterotrimers with wild-type TRIM5α, accounting for the observed dominant-negative activity of the mutant protein. Trimerization potentially allows TRIM5α to interact with threefold pseudosymmetrical structures on retroviral capsids.

Original languageEnglish (US)
Pages (from-to)14446-14450
Number of pages5
JournalJournal of Virology
Volume79
Issue number22
DOIs
StatePublished - Nov 2005
Externally publishedYes

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capsid
Capsid
Retroviridae
mutants
Mutant Proteins
proteins
Proteins
B30.2-SPRY Domain

ASJC Scopus subject areas

  • Immunology

Cite this

Mische, C. C., Javanbakht, H., Song, B., Diaz-Griffero, F., Stremlau, M., Strack, B., ... Sodroski, J. (2005). Retroviral restriction factor TRIM5α is a trimer. Journal of Virology, 79(22), 14446-14450. https://doi.org/10.1128/JVI.79.22.14446-14450.2005

Retroviral restriction factor TRIM5α is a trimer. / Mische, Claudia C.; Javanbakht, Hassan; Song, Byeongwoon; Diaz-Griffero, Felipe; Stremlau, Matthew; Strack, Bettina; Si, Zhihai; Sodroski, Joseph.

In: Journal of Virology, Vol. 79, No. 22, 11.2005, p. 14446-14450.

Research output: Contribution to journalArticle

Mische, CC, Javanbakht, H, Song, B, Diaz-Griffero, F, Stremlau, M, Strack, B, Si, Z & Sodroski, J 2005, 'Retroviral restriction factor TRIM5α is a trimer', Journal of Virology, vol. 79, no. 22, pp. 14446-14450. https://doi.org/10.1128/JVI.79.22.14446-14450.2005
Mische CC, Javanbakht H, Song B, Diaz-Griffero F, Stremlau M, Strack B et al. Retroviral restriction factor TRIM5α is a trimer. Journal of Virology. 2005 Nov;79(22):14446-14450. https://doi.org/10.1128/JVI.79.22.14446-14450.2005
Mische, Claudia C. ; Javanbakht, Hassan ; Song, Byeongwoon ; Diaz-Griffero, Felipe ; Stremlau, Matthew ; Strack, Bettina ; Si, Zhihai ; Sodroski, Joseph. / Retroviral restriction factor TRIM5α is a trimer. In: Journal of Virology. 2005 ; Vol. 79, No. 22. pp. 14446-14450.
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