Retinoic acid regulates, in vitro, the two normal pathways of differentiation of human laryngeal keratinocytes

M. G. Mendelsohn, T. P. Dilorenzo, A. L. Abramson, B. M. Steinberg

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Abstract

We have investigated the regulation of the two normal differentiation pathways followed by laryngeal epithelium. Using a tissue culture system that permits growth of cells at the air-liquid interface in serum-free medium, we found that modulating the concentration of retinoic acid is sufficient to determine which pathway is used. At 10-8M retinoic acid, the cells form a stratified squamous epithelium which expresses the differentiation-specific keratin K13. At 10-7 M retinoic acid, the cells form a ciliated pseudostratified epithelium, with no expression of K13. These results are distinct from those seen with foreskin keratinocytes, which have only a single pathway of normal differentiation.

Original languageEnglish (US)
Pages (from-to)137-141
Number of pages5
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume27
Issue number2
DOIs
Publication statusPublished - Jan 1 1991

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ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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