Retinoic acid regulates, in vitro, the two normal pathways of differentiation of human laryngeal keratinocytes

M. G. Mendelsohn; T. P. Dilorenzo; A. L. Abramson; B. M. Steinberg

doi:10.1007/BF02630999

Retinoic acid regulates, in vitro, the two normal pathways of differentiation of human laryngeal keratinocytes

M. G. Mendelsohn, T. P. Dilorenzo, A. L. Abramson, B. M. Steinberg

Microbiology & Immunology

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Abstract

We have investigated the regulation of the two normal differentiation pathways followed by laryngeal epithelium. Using a tissue culture system that permits growth of cells at the air-liquid interface in serum-free medium, we found that modulating the concentration of retinoic acid is sufficient to determine which pathway is used. At 10^-8M retinoic acid, the cells form a stratified squamous epithelium which expresses the differentiation-specific keratin K13. At 10^-7 M retinoic acid, the cells form a ciliated pseudostratified epithelium, with no expression of K13. These results are distinct from those seen with foreskin keratinocytes, which have only a single pathway of normal differentiation.

Original language	English (US)
Pages (from-to)	137-141
Number of pages	5
Journal	In Vitro Cellular and Developmental Biology - Animal
Volume	27
Issue number	2
DOIs	https://doi.org/10.1007/BF02630999
State	Published - 1991

ASJC Scopus subject areas

Developmental Biology
Clinical Biochemistry
Cell Biology

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abstract = "We have investigated the regulation of the two normal differentiation pathways followed by laryngeal epithelium. Using a tissue culture system that permits growth of cells at the air-liquid interface in serum-free medium, we found that modulating the concentration of retinoic acid is sufficient to determine which pathway is used. At 10-8M retinoic acid, the cells form a stratified squamous epithelium which expresses the differentiation-specific keratin K13. At 10-7 M retinoic acid, the cells form a ciliated pseudostratified epithelium, with no expression of K13. These results are distinct from those seen with foreskin keratinocytes, which have only a single pathway of normal differentiation.",

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AU - Steinberg, B. M.

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Retinoic acid regulates, in vitro, the two normal pathways of differentiation of human laryngeal keratinocytes

Abstract

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