Results of human papillomavirus DNA testing with the Hybrid Capture 2 assay are reproducible

Philip E. Castle, Attila T. Lorincz, Iwona Mielzynska-Lohnas, David R. Scott, Andrew G. Glass, Mark E. Sherman, John E. Schussler, Mark Schiffman

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Abstract

Reproducibility of the Hybrid Capture 2 Test (HC 2) for human papillomavirus (HPV) DNA detection was evaluated by assaying frozen cervical specimens in 1997 and again in 2001 from 1,775 women with normal cervical cytology. Using a cutoff point of 1.0 pg of HPV DNA/ml between a negative and a positive test result, the result of the kappa test for agreement was 0.72 (a kappa value of >0.60 is considered good agreement). Using cutoff points of 1.0 and 10.0 pg/ml between negative and low positive and between low positive and high positive, respectively, the kappa was 0.68 and the linear-weighted kappa was 0.76. The results of this study indicate that HC 2 testing is reproducible even among cytologically normal women with low test values.

Original languageEnglish (US)
Pages (from-to)1088-1090
Number of pages3
JournalJournal of Clinical Microbiology
Volume40
Issue number3
DOIs
StatePublished - Mar 20 2002
Externally publishedYes

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ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Castle, P. E., Lorincz, A. T., Mielzynska-Lohnas, I., Scott, D. R., Glass, A. G., Sherman, M. E., Schussler, J. E., & Schiffman, M. (2002). Results of human papillomavirus DNA testing with the Hybrid Capture 2 assay are reproducible. Journal of Clinical Microbiology, 40(3), 1088-1090. https://doi.org/10.1128/JCM.40.3.1088-1090.2002