Results of human papillomavirus DNA testing with the Hybrid Capture 2 assay are reproducible

Philip E. Castle, Attila T. Lorincz, Iwona Mielzynska-Lohnas, David R. Scott, Andrew G. Glass, Mark E. Sherman, John E. Schussler, Mark Schiffman

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Reproducibility of the Hybrid Capture 2 Test (HC 2) for human papillomavirus (HPV) DNA detection was evaluated by assaying frozen cervical specimens in 1997 and again in 2001 from 1,775 women with normal cervical cytology. Using a cutoff point of 1.0 pg of HPV DNA/ml between a negative and a positive test result, the result of the kappa test for agreement was 0.72 (a kappa value of >0.60 is considered good agreement). Using cutoff points of 1.0 and 10.0 pg/ml between negative and low positive and between low positive and high positive, respectively, the kappa was 0.68 and the linear-weighted kappa was 0.76. The results of this study indicate that HC 2 testing is reproducible even among cytologically normal women with low test values.

Original languageEnglish (US)
Pages (from-to)1088-1090
Number of pages3
JournalJournal of Clinical Microbiology
Volume40
Issue number3
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Human Papillomavirus DNA Tests
DNA
Cell Biology

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Castle, P. E., Lorincz, A. T., Mielzynska-Lohnas, I., Scott, D. R., Glass, A. G., Sherman, M. E., ... Schiffman, M. (2002). Results of human papillomavirus DNA testing with the Hybrid Capture 2 assay are reproducible. Journal of Clinical Microbiology, 40(3), 1088-1090. https://doi.org/10.1128/JCM.40.3.1088-1090.2002

Results of human papillomavirus DNA testing with the Hybrid Capture 2 assay are reproducible. / Castle, Philip E.; Lorincz, Attila T.; Mielzynska-Lohnas, Iwona; Scott, David R.; Glass, Andrew G.; Sherman, Mark E.; Schussler, John E.; Schiffman, Mark.

In: Journal of Clinical Microbiology, Vol. 40, No. 3, 2002, p. 1088-1090.

Research output: Contribution to journalArticle

Castle, PE, Lorincz, AT, Mielzynska-Lohnas, I, Scott, DR, Glass, AG, Sherman, ME, Schussler, JE & Schiffman, M 2002, 'Results of human papillomavirus DNA testing with the Hybrid Capture 2 assay are reproducible', Journal of Clinical Microbiology, vol. 40, no. 3, pp. 1088-1090. https://doi.org/10.1128/JCM.40.3.1088-1090.2002
Castle, Philip E. ; Lorincz, Attila T. ; Mielzynska-Lohnas, Iwona ; Scott, David R. ; Glass, Andrew G. ; Sherman, Mark E. ; Schussler, John E. ; Schiffman, Mark. / Results of human papillomavirus DNA testing with the Hybrid Capture 2 assay are reproducible. In: Journal of Clinical Microbiology. 2002 ; Vol. 40, No. 3. pp. 1088-1090.
@article{ecafe1ccadf84c898dac87e13ebac4b0,
title = "Results of human papillomavirus DNA testing with the Hybrid Capture 2 assay are reproducible",
abstract = "Reproducibility of the Hybrid Capture 2 Test (HC 2) for human papillomavirus (HPV) DNA detection was evaluated by assaying frozen cervical specimens in 1997 and again in 2001 from 1,775 women with normal cervical cytology. Using a cutoff point of 1.0 pg of HPV DNA/ml between a negative and a positive test result, the result of the kappa test for agreement was 0.72 (a kappa value of >0.60 is considered good agreement). Using cutoff points of 1.0 and 10.0 pg/ml between negative and low positive and between low positive and high positive, respectively, the kappa was 0.68 and the linear-weighted kappa was 0.76. The results of this study indicate that HC 2 testing is reproducible even among cytologically normal women with low test values.",
author = "Castle, {Philip E.} and Lorincz, {Attila T.} and Iwona Mielzynska-Lohnas and Scott, {David R.} and Glass, {Andrew G.} and Sherman, {Mark E.} and Schussler, {John E.} and Mark Schiffman",
year = "2002",
doi = "10.1128/JCM.40.3.1088-1090.2002",
language = "English (US)",
volume = "40",
pages = "1088--1090",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Results of human papillomavirus DNA testing with the Hybrid Capture 2 assay are reproducible

AU - Castle, Philip E.

AU - Lorincz, Attila T.

AU - Mielzynska-Lohnas, Iwona

AU - Scott, David R.

AU - Glass, Andrew G.

AU - Sherman, Mark E.

AU - Schussler, John E.

AU - Schiffman, Mark

PY - 2002

Y1 - 2002

N2 - Reproducibility of the Hybrid Capture 2 Test (HC 2) for human papillomavirus (HPV) DNA detection was evaluated by assaying frozen cervical specimens in 1997 and again in 2001 from 1,775 women with normal cervical cytology. Using a cutoff point of 1.0 pg of HPV DNA/ml between a negative and a positive test result, the result of the kappa test for agreement was 0.72 (a kappa value of >0.60 is considered good agreement). Using cutoff points of 1.0 and 10.0 pg/ml between negative and low positive and between low positive and high positive, respectively, the kappa was 0.68 and the linear-weighted kappa was 0.76. The results of this study indicate that HC 2 testing is reproducible even among cytologically normal women with low test values.

AB - Reproducibility of the Hybrid Capture 2 Test (HC 2) for human papillomavirus (HPV) DNA detection was evaluated by assaying frozen cervical specimens in 1997 and again in 2001 from 1,775 women with normal cervical cytology. Using a cutoff point of 1.0 pg of HPV DNA/ml between a negative and a positive test result, the result of the kappa test for agreement was 0.72 (a kappa value of >0.60 is considered good agreement). Using cutoff points of 1.0 and 10.0 pg/ml between negative and low positive and between low positive and high positive, respectively, the kappa was 0.68 and the linear-weighted kappa was 0.76. The results of this study indicate that HC 2 testing is reproducible even among cytologically normal women with low test values.

UR - http://www.scopus.com/inward/record.url?scp=0036197334&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036197334&partnerID=8YFLogxK

U2 - 10.1128/JCM.40.3.1088-1090.2002

DO - 10.1128/JCM.40.3.1088-1090.2002

M3 - Article

C2 - 11880448

AN - SCOPUS:0036197334

VL - 40

SP - 1088

EP - 1090

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 3

ER -