Restriction endonuclease mapping of the Escherichia coli K12 chromosome in the vicinity of the ilv genes

Geoffrey J. Childs, Hisako Ohtsubo, Eiichi Ohtsubo, Frank Sonnenberg, Martin Freundlich

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We have determined the EcoRI endonuclease fragment map of the λh80dilv chromosome as well as the EcoRI, HindIII and BamI cleavage patterns of the Escherichia coli chromosome around the ilv genes on F310, F312 and λh80dilv. Comparison of fragment maps from F-prime ilv DNAs with λh80dilv DNA shows the orientation of the bacterial genes on the λh80dilv chromosome. These data, taken together with the results obtained by hybridization of ilv messenger RNA to λh80dilv DNA digested with strand-specific exonucleases, strongly suggest that the ilvOADGE operon is transcribed from a promoter-proximal ilvE to the ilvO gene. This is opposite to the direction of transcription previously reported. The nature of the cis-dominant operator-like mutations that define ilvO remains to be clarified. Hybridization data obtained with EcoRI fragments of the ilv gene cluster indicate that these fragments may be valuable for studying transcription of the individual ilv operons. A model for the fusion of parental F-prime factors F′-lac att80trp (F′trp/7) and F′-ilv (F16) is proposed in which recombination and inversion of bacterial DNA between the two copies of the F DNA sequences at position 2.8F to 8.5F allows the formation of a λh80dilv chromosome with the proposed structure.

Original languageEnglish (US)
Pages (from-to)175-193
Number of pages19
JournalJournal of Molecular Biology
Volume117
Issue number1
DOIs
StatePublished - Nov 25 1977
Externally publishedYes

Fingerprint

Escherichia coli K12
Restriction Mapping
Chromosomes
Operon
Genes
DNA
Deoxyribonuclease EcoRI
Bacterial Genes
Bacterial DNA
Exonucleases
Multigene Family
Genetic Recombination
Escherichia coli
Messenger RNA
Mutation

ASJC Scopus subject areas

  • Virology

Cite this

Restriction endonuclease mapping of the Escherichia coli K12 chromosome in the vicinity of the ilv genes. / Childs, Geoffrey J.; Ohtsubo, Hisako; Ohtsubo, Eiichi; Sonnenberg, Frank; Freundlich, Martin.

In: Journal of Molecular Biology, Vol. 117, No. 1, 25.11.1977, p. 175-193.

Research output: Contribution to journalArticle

Childs, Geoffrey J. ; Ohtsubo, Hisako ; Ohtsubo, Eiichi ; Sonnenberg, Frank ; Freundlich, Martin. / Restriction endonuclease mapping of the Escherichia coli K12 chromosome in the vicinity of the ilv genes. In: Journal of Molecular Biology. 1977 ; Vol. 117, No. 1. pp. 175-193.
@article{77f1341471e74f4d8a2b6a9e6a20689f,
title = "Restriction endonuclease mapping of the Escherichia coli K12 chromosome in the vicinity of the ilv genes",
abstract = "We have determined the EcoRI endonuclease fragment map of the λh80dilv chromosome as well as the EcoRI, HindIII and BamI cleavage patterns of the Escherichia coli chromosome around the ilv genes on F310, F312 and λh80dilv. Comparison of fragment maps from F-prime ilv DNAs with λh80dilv DNA shows the orientation of the bacterial genes on the λh80dilv chromosome. These data, taken together with the results obtained by hybridization of ilv messenger RNA to λh80dilv DNA digested with strand-specific exonucleases, strongly suggest that the ilvOADGE operon is transcribed from a promoter-proximal ilvE to the ilvO gene. This is opposite to the direction of transcription previously reported. The nature of the cis-dominant operator-like mutations that define ilvO remains to be clarified. Hybridization data obtained with EcoRI fragments of the ilv gene cluster indicate that these fragments may be valuable for studying transcription of the individual ilv operons. A model for the fusion of parental F-prime factors F′-lac att80trp (F′trp/7) and F′-ilv (F16) is proposed in which recombination and inversion of bacterial DNA between the two copies of the F DNA sequences at position 2.8F to 8.5F allows the formation of a λh80dilv chromosome with the proposed structure.",
author = "Childs, {Geoffrey J.} and Hisako Ohtsubo and Eiichi Ohtsubo and Frank Sonnenberg and Martin Freundlich",
year = "1977",
month = "11",
day = "25",
doi = "10.1016/0022-2836(77)90030-4",
language = "English (US)",
volume = "117",
pages = "175--193",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Restriction endonuclease mapping of the Escherichia coli K12 chromosome in the vicinity of the ilv genes

AU - Childs, Geoffrey J.

AU - Ohtsubo, Hisako

AU - Ohtsubo, Eiichi

AU - Sonnenberg, Frank

AU - Freundlich, Martin

PY - 1977/11/25

Y1 - 1977/11/25

N2 - We have determined the EcoRI endonuclease fragment map of the λh80dilv chromosome as well as the EcoRI, HindIII and BamI cleavage patterns of the Escherichia coli chromosome around the ilv genes on F310, F312 and λh80dilv. Comparison of fragment maps from F-prime ilv DNAs with λh80dilv DNA shows the orientation of the bacterial genes on the λh80dilv chromosome. These data, taken together with the results obtained by hybridization of ilv messenger RNA to λh80dilv DNA digested with strand-specific exonucleases, strongly suggest that the ilvOADGE operon is transcribed from a promoter-proximal ilvE to the ilvO gene. This is opposite to the direction of transcription previously reported. The nature of the cis-dominant operator-like mutations that define ilvO remains to be clarified. Hybridization data obtained with EcoRI fragments of the ilv gene cluster indicate that these fragments may be valuable for studying transcription of the individual ilv operons. A model for the fusion of parental F-prime factors F′-lac att80trp (F′trp/7) and F′-ilv (F16) is proposed in which recombination and inversion of bacterial DNA between the two copies of the F DNA sequences at position 2.8F to 8.5F allows the formation of a λh80dilv chromosome with the proposed structure.

AB - We have determined the EcoRI endonuclease fragment map of the λh80dilv chromosome as well as the EcoRI, HindIII and BamI cleavage patterns of the Escherichia coli chromosome around the ilv genes on F310, F312 and λh80dilv. Comparison of fragment maps from F-prime ilv DNAs with λh80dilv DNA shows the orientation of the bacterial genes on the λh80dilv chromosome. These data, taken together with the results obtained by hybridization of ilv messenger RNA to λh80dilv DNA digested with strand-specific exonucleases, strongly suggest that the ilvOADGE operon is transcribed from a promoter-proximal ilvE to the ilvO gene. This is opposite to the direction of transcription previously reported. The nature of the cis-dominant operator-like mutations that define ilvO remains to be clarified. Hybridization data obtained with EcoRI fragments of the ilv gene cluster indicate that these fragments may be valuable for studying transcription of the individual ilv operons. A model for the fusion of parental F-prime factors F′-lac att80trp (F′trp/7) and F′-ilv (F16) is proposed in which recombination and inversion of bacterial DNA between the two copies of the F DNA sequences at position 2.8F to 8.5F allows the formation of a λh80dilv chromosome with the proposed structure.

UR - http://www.scopus.com/inward/record.url?scp=0017716365&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017716365&partnerID=8YFLogxK

U2 - 10.1016/0022-2836(77)90030-4

DO - 10.1016/0022-2836(77)90030-4

M3 - Article

C2 - 340701

AN - SCOPUS:0017716365

VL - 117

SP - 175

EP - 193

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 1

ER -