Restoration of circadian behavioural rhythms by gene transfer in Drosophila

Thaddeus A. Bargiello, F. Rob Jackson, Michael W. Young

Research output: Contribution to journalArticle

246 Citations (Scopus)

Abstract

The per locus of Drosophila melanogaster has a fundamental role in the construction or maintenance of a biological clock. Three classes of per mutations have been identified: Perl mutants have circadian behavioural rhythms with a 29-h rather than a 24-h period, pers mutants have short-period rhythms of 19 h, and per0 mutants have no detectable circadian rhythms1-4. Each of these mutations has a corresponding influence on the 55-s periodicity of male courtship song 5. Long-and short-period circadian rhythm phenotypes can also be obtained by altering the dosage of the wild-type gene4: For example, females carrying only one dose of this X-linked gene have circadian rhythms with periodicities about 1 h longer than those carrying two doses. In a previous report6, cloned DNA was used to localize several chromosomal rearrangement breakpoints that alter per locus function. The rearrangements all affected a 7-kilobase (kb) interval that encodes a 4.5-kb poly(A)+ RNA. We report here that when a 7.1-kb fragment from a per+ fly, including the sequences encoding the 4.5-kb transcript, is introduced into the genome of a per0 (arrhythmic) fly by P element-mediated transformation, circadian rhythmicity of behaviour such as eclosion and locomotor activity is restored. The transforming DNA complements per locus deletions and is transcribed, forming a single 4.5-kb poly(A)+ RNA comparableto that produced by wild-type flies.

Original languageEnglish (US)
Pages (from-to)752-754
Number of pages3
JournalNature
Volume312
Issue number5996
DOIs
StatePublished - 1984
Externally publishedYes

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Periodicity
Circadian Rhythm
Drosophila
Diptera
Biological Clocks
Genes
Courtship
X-Linked Genes
Messenger RNA
Mutation
DNA
Locomotion
Music
Drosophila melanogaster
Genome
Phenotype

ASJC Scopus subject areas

  • General

Cite this

Bargiello, T. A., Jackson, F. R., & Young, M. W. (1984). Restoration of circadian behavioural rhythms by gene transfer in Drosophila. Nature, 312(5996), 752-754. https://doi.org/10.1038/312752a0

Restoration of circadian behavioural rhythms by gene transfer in Drosophila. / Bargiello, Thaddeus A.; Jackson, F. Rob; Young, Michael W.

In: Nature, Vol. 312, No. 5996, 1984, p. 752-754.

Research output: Contribution to journalArticle

Bargiello, TA, Jackson, FR & Young, MW 1984, 'Restoration of circadian behavioural rhythms by gene transfer in Drosophila', Nature, vol. 312, no. 5996, pp. 752-754. https://doi.org/10.1038/312752a0
Bargiello, Thaddeus A. ; Jackson, F. Rob ; Young, Michael W. / Restoration of circadian behavioural rhythms by gene transfer in Drosophila. In: Nature. 1984 ; Vol. 312, No. 5996. pp. 752-754.
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