Resonance Raman studies of bovine metarhodopsin I and metarhodopsin II

A. G. Doukas, B. Aton, Robert Callender, T. G. Ebrey

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

The resonance Raman spectra of bovine metarhodopsin I and metarhodopsin II have been measured. The spectra are compared with model chromophore resonance Raman data. It was found that metarhodopsin I is linked to opsin via a protonated Schiff base linkage, whereas metarhodopsin II is linked by an unprotonated Schiff base. A recent suggestion that the chromophore of metarhodopsin II is retinal is explicitly disproved. The chromophores of both metarhodopsins are found to have an essentially all-trans conformation. The basic mechanism for color regulation in both forms appears to be electron delocalization. The data tend to support the model of cis-trans isomerization as the primary mechanism for vision. Also, the conclusions and inferences of this work on energy uses and storage by rhodopsin in neural generation are discussed.

Original languageEnglish (US)
Pages (from-to)2430-2435
Number of pages6
JournalBiochemistry
Volume17
Issue number12
StatePublished - 1978
Externally publishedYes

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Chromophores
Schiff Bases
Opsins
Rhodopsin
Isomerization
Conformations
metarhodopsins
Raman scattering
Color
Electrons

ASJC Scopus subject areas

  • Biochemistry

Cite this

Doukas, A. G., Aton, B., Callender, R., & Ebrey, T. G. (1978). Resonance Raman studies of bovine metarhodopsin I and metarhodopsin II. Biochemistry, 17(12), 2430-2435.

Resonance Raman studies of bovine metarhodopsin I and metarhodopsin II. / Doukas, A. G.; Aton, B.; Callender, Robert; Ebrey, T. G.

In: Biochemistry, Vol. 17, No. 12, 1978, p. 2430-2435.

Research output: Contribution to journalArticle

Doukas, AG, Aton, B, Callender, R & Ebrey, TG 1978, 'Resonance Raman studies of bovine metarhodopsin I and metarhodopsin II', Biochemistry, vol. 17, no. 12, pp. 2430-2435.
Doukas, A. G. ; Aton, B. ; Callender, Robert ; Ebrey, T. G. / Resonance Raman studies of bovine metarhodopsin I and metarhodopsin II. In: Biochemistry. 1978 ; Vol. 17, No. 12. pp. 2430-2435.
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