Requirements for capsid-binding and an effector function in TRIMCyp-mediated restriction of HIV-1

Felipe Diaz-Griffero, Nick Vandegraaff, Yuan Li, Kathleen McGee-Estrada, Matthew Stremlau, Sohanya Welikala, Zhihai Si, Alan Engelman, Joseph Sodroski

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

In owl monkeys, a retrotransposition event replaced the gene encoding the retroviral restriction factor TRIM5α with one encoding TRIMCyp, a fusion between the RING, B-box 2 and coiled-coil domains of TRIM5 and cyclophilin A. TRIMCyp restricts human immunodeficiency virus (HIV-1) infection by a mechanism dependent on the interaction of the cyclophilin A moiety and the HIV-1 capsid protein. Here, we show that infection by retroviruses other than HIV-1 can be restricted by TRIMCyp, providing an explanation for the evolutionary retention of the TRIMCyp gene in owl monkey lineages. The TRIMCyp-mediated block to HIV-1 infection occurs before the earliest step of reverse transcription. TRIMCyp-mediated restriction involves at least two functions: (1) capsid binding, which occurs most efficiently for trimeric TRIMCyp proteins that retain the coiled-coil and cyclophilin A domains, and (2) an effector function that depends upon the B-box 2 domain.

Original languageEnglish (US)
Pages (from-to)404-419
Number of pages16
JournalVirology
Volume351
Issue number2
DOIs
StatePublished - Aug 1 2006
Externally publishedYes

Fingerprint

Capsid
Cyclophilin A
HIV-1
Aotidae
HIV Infections
Retroviridae Infections
Capsid Proteins
Genes
Reverse Transcription
Proteins

Keywords

  • Capsid
  • Cyclophilin
  • HIV
  • Restriction factors
  • Retrovirus
  • Reverse transcription
  • Tropism
  • Uncoating

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Diaz-Griffero, F., Vandegraaff, N., Li, Y., McGee-Estrada, K., Stremlau, M., Welikala, S., ... Sodroski, J. (2006). Requirements for capsid-binding and an effector function in TRIMCyp-mediated restriction of HIV-1. Virology, 351(2), 404-419. https://doi.org/10.1016/j.virol.2006.03.023

Requirements for capsid-binding and an effector function in TRIMCyp-mediated restriction of HIV-1. / Diaz-Griffero, Felipe; Vandegraaff, Nick; Li, Yuan; McGee-Estrada, Kathleen; Stremlau, Matthew; Welikala, Sohanya; Si, Zhihai; Engelman, Alan; Sodroski, Joseph.

In: Virology, Vol. 351, No. 2, 01.08.2006, p. 404-419.

Research output: Contribution to journalArticle

Diaz-Griffero, F, Vandegraaff, N, Li, Y, McGee-Estrada, K, Stremlau, M, Welikala, S, Si, Z, Engelman, A & Sodroski, J 2006, 'Requirements for capsid-binding and an effector function in TRIMCyp-mediated restriction of HIV-1', Virology, vol. 351, no. 2, pp. 404-419. https://doi.org/10.1016/j.virol.2006.03.023
Diaz-Griffero, Felipe ; Vandegraaff, Nick ; Li, Yuan ; McGee-Estrada, Kathleen ; Stremlau, Matthew ; Welikala, Sohanya ; Si, Zhihai ; Engelman, Alan ; Sodroski, Joseph. / Requirements for capsid-binding and an effector function in TRIMCyp-mediated restriction of HIV-1. In: Virology. 2006 ; Vol. 351, No. 2. pp. 404-419.
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