Requirement of transcription factor NFAT in developing atrial myocardium

William Schubert, Xiao Yong Yang, Teddy T.C. Yang, Stephen M. Factor, Michael P. Lisanti, Jeffery D. Molkentin, Mercedes Rincón, Chi Wing Chow

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Nuclear factor of activated T cell (NFAT) is a ubiquitous regulator involved in multiple biological processes. Here, we demonstrate that NFAT is temporally required in the developing atrial myocardium between embryonic day 14 and PO (birth). Inhibition of NFAT activity by conditional expression of dominant-negative NFAT causes thinning of the atrial myocardium. The thin myocardium exhibits severe sarcomere disorganization and reduced expression of cardiac troponin-I (cTnI) and cardiac troponin-T (cTnT). Promoter analysis indicates that NFAT binds to and regulates transcription of the cTnI and the cTnT genes. Thus, regulation of cytoskeletal protein gene expression by NFAT may be important for the structural architecture of the developing atrial myocardium.

Original languageEnglish (US)
Pages (from-to)861-874
Number of pages14
JournalJournal of Cell Biology
Volume161
Issue number5
DOIs
Publication statusPublished - Jun 9 2003

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Keywords

  • Cardiac myocytes
  • Heart development
  • Inducible transgenic mice
  • Sarcomeric proteins
  • Troponin complex

ASJC Scopus subject areas

  • Cell Biology

Cite this

Schubert, W., Yang, X. Y., Yang, T. T. C., Factor, S. M., Lisanti, M. P., Molkentin, J. D., ... Chow, C. W. (2003). Requirement of transcription factor NFAT in developing atrial myocardium. Journal of Cell Biology, 161(5), 861-874. https://doi.org/10.1083/jcb.200301058