Requirement of histone deacetylase activity for signaling by STAT1

Lidija Klampfer, Jie Huang, Laurie Anne Swaby, Leonard H. Augenlicht

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

STAT1 is a transcription factor that plays a crucial role in signaling by interferons (IFNs). In this study we demonstrated that inhibitors of histone deacetylase (HDAC) activity, butyrate, trichostatin A, and suberoylanilide hydroxamic acid, prevented IFNγ-induced JAK1 activation, STAT1 phosphorylation, its nuclear translocation, and STAT1-dependent gene activation Furthermore, we showed that silencing of HDAC1, HDAC2, and HDAC3 through RNA interference markedly decreased IFNγ-driven gene activation and that overexpression of HDAC1, HDAC2, and HDAC3 enhanced STAT1-dependent transcriptional activity. Our data therefore established the essential role of deacetylase activity in STAT1 signaling. Induction of IRF-1 by IFNγ requires functional STAT1 signaling and was abrogated by butyrate, trichostatin A, suberoylanilide hydroxamic acid, and STAT1 small interfering RNA. In contrast, silencing of STAT1 did not interfere with IFNγ-induced expression of STAT2 and caspase-7, and HDAC inhibitors did not preclude IFNγ-induced expression of STAT1, STAT2, and caspase-7, suggesting that HDAC inhibitors impede the expression of IFNγ target genes whose expression depends on STAT1 but do not interfere with STAT1-independent signaling by IFNγ. Finally, we showed that inhibitors of deacetylase activity sensitized colon cancer cells to IFNγ-induced apoptosis through cooperative negative regulation of Bcl-x expression, demonstrating that interruption of the balance between STAT1-dependent and STAT1-independent signaling significantly alters the biological activity of IFNγ.

Original languageEnglish (US)
Pages (from-to)30358-30368
Number of pages11
JournalJournal of Biological Chemistry
Volume279
Issue number29
DOIs
StatePublished - Jul 16 2004

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Histone Deacetylases
Interferons
Histone Deacetylase Inhibitors
trichostatin A
Caspase 7
Butyrates
Chemical activation
Transcriptional Activation
Genes
Phosphorylation
RNA Interference
Bioactivity
Gene expression
Colonic Neoplasms
Small Interfering RNA
Transcription Factors
Cells
RNA
Apoptosis
Gene Expression

ASJC Scopus subject areas

  • Biochemistry

Cite this

Requirement of histone deacetylase activity for signaling by STAT1. / Klampfer, Lidija; Huang, Jie; Swaby, Laurie Anne; Augenlicht, Leonard H.

In: Journal of Biological Chemistry, Vol. 279, No. 29, 16.07.2004, p. 30358-30368.

Research output: Contribution to journalArticle

Klampfer, Lidija ; Huang, Jie ; Swaby, Laurie Anne ; Augenlicht, Leonard H. / Requirement of histone deacetylase activity for signaling by STAT1. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 29. pp. 30358-30368.
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