Requirement for prolactin during cell cycle regulated gene expression in cloned T-lymphocytes

Charles V. Clevenger, Amy L. Sillman, Joan Hanley-Hyde, Michael B. Prystowsky

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

The neuroendocrine hormone PRL acts as a progression factor during interleukin-2 (IL2) stimulated lymphocyte proliferation. Since the sequential expression of cell cycle regulated genes occurs during this process, we examined the contribution of IL2 and PRL to specific RNA accumulation. Stimulation of the cloned T cell line L2 with IL2 and PRL induced the sequential expression of Interferon regulatory factor-1, c-myc, proliferating cell nuclear antigen, thymidine kinase, cyclin B, and histone H3. Stimulation of L2 cells with PRL alone, however, induced only the expression of Interferon regulatory factor-1. Depletion of PRL, through the use of an anti-PRL antiserum, inhibited IL2 driven proliferation and the expression of cyclin B and histone H3. These results demonstrate that PRL may regulate T cell proliferation by enhancing the expression of some genes necessary for entry into S-phase.

Original languageEnglish (US)
Pages (from-to)3216-3222
Number of pages7
JournalEndocrinology
Volume130
Issue number6
StatePublished - Jun 1992
Externally publishedYes

Fingerprint

cdc Genes
Prolactin
Interleukin-2
Interferon Regulatory Factor-1
Cyclin B
T-Lymphocytes
Gene Expression
Histones
Thymidine Kinase
Proliferating Cell Nuclear Antigen
S Phase
Immune Sera
Cell Proliferation
Hormones
Lymphocytes
RNA
Cell Line

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Requirement for prolactin during cell cycle regulated gene expression in cloned T-lymphocytes. / Clevenger, Charles V.; Sillman, Amy L.; Hanley-Hyde, Joan; Prystowsky, Michael B.

In: Endocrinology, Vol. 130, No. 6, 06.1992, p. 3216-3222.

Research output: Contribution to journalArticle

Clevenger, CV, Sillman, AL, Hanley-Hyde, J & Prystowsky, MB 1992, 'Requirement for prolactin during cell cycle regulated gene expression in cloned T-lymphocytes', Endocrinology, vol. 130, no. 6, pp. 3216-3222.
Clevenger, Charles V. ; Sillman, Amy L. ; Hanley-Hyde, Joan ; Prystowsky, Michael B. / Requirement for prolactin during cell cycle regulated gene expression in cloned T-lymphocytes. In: Endocrinology. 1992 ; Vol. 130, No. 6. pp. 3216-3222.
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