Reproductive Aging is Associated with Altered Gene Expression in Human Luteinized Granulosa Cells

Joshua M. Hurwitz, Sangita K. Jindal, Keri Greenseid, Dara Berger, Andrew Brooks, Nanette Santoro, Lubna Pal

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Declining reproductive success with aging is attributable to qualitative and quantitative deterioration in oocytes, which are nurtured by granulosa cells (GCs). This prospective study assesses whether reproductive aging is accompanied by differential gene expression in luteinized GCs from in vitro fertilization (IVF) patients. Women with nonovarian infertility etiologies were categorized as younger (≤30, n = 3) or older (≥40, n = 3). During oocyte retrieval, mural GCs were isolated; messenger RNA (mRNA) was extracted and transcribed for complementary DNA (cDNA) microarray analysis. Differential gene expression was confirmed by real-time polymerase chain reaction (PCR). Analysis revealed 120 genes were differentially expressed. Three genes were upregulated and 117 were downregulated (including interleukin [IL]-1β, IL-1R2, and IL-6R) in GCs of older versus younger patients. Our data provide evidence of downregulation in IL-1 and IL-6 gene families in luteinized GCs with advancing age. Given previously recognized roles for the IL gene family in folliculogenesis and ovulation, our findings may partly explain ovulatory and luteal dysfunctions associated with reproductive aging.

Original languageEnglish (US)
Pages (from-to)56-67
Number of pages12
JournalReproductive Sciences
Volume17
Issue number1
DOIs
StatePublished - Jan 2010

Fingerprint

Granulosa Cells
Interleukins
Gene Expression
Interleukin-1
Genes
Down-Regulation
Oocyte Retrieval
Corpus Luteum
Fertilization in Vitro
Microarray Analysis
Ovulation
Oligonucleotide Array Sequence Analysis
Infertility
Oocytes
Real-Time Polymerase Chain Reaction
Interleukin-6
Complementary DNA
Prospective Studies
Messenger RNA

Keywords

  • Gene expression
  • Granulosa cells
  • Interleukin-1
  • Interleukin-6.
  • Reproductive aging

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Medicine(all)

Cite this

Reproductive Aging is Associated with Altered Gene Expression in Human Luteinized Granulosa Cells. / Hurwitz, Joshua M.; Jindal, Sangita K.; Greenseid, Keri; Berger, Dara; Brooks, Andrew; Santoro, Nanette; Pal, Lubna.

In: Reproductive Sciences, Vol. 17, No. 1, 01.2010, p. 56-67.

Research output: Contribution to journalArticle

Hurwitz, Joshua M. ; Jindal, Sangita K. ; Greenseid, Keri ; Berger, Dara ; Brooks, Andrew ; Santoro, Nanette ; Pal, Lubna. / Reproductive Aging is Associated with Altered Gene Expression in Human Luteinized Granulosa Cells. In: Reproductive Sciences. 2010 ; Vol. 17, No. 1. pp. 56-67.
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