Reproductive Aging is Associated with Altered Gene Expression in Human Luteinized Granulosa Cells

Joshua M. Hurwitz, Sangita Jindal, Keri Greenseid, Dara Berger, Andrew Brooks, Nanette Santoro, Lubna Pal

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Declining reproductive success with aging is attributable to qualitative and quantitative deterioration in oocytes, which are nurtured by granulosa cells (GCs). This prospective study assesses whether reproductive aging is accompanied by differential gene expression in luteinized GCs from in vitro fertilization (IVF) patients. Women with nonovarian infertility etiologies were categorized as younger (≤30, n = 3) or older (≥40, n = 3). During oocyte retrieval, mural GCs were isolated; messenger RNA (mRNA) was extracted and transcribed for complementary DNA (cDNA) microarray analysis. Differential gene expression was confirmed by real-time polymerase chain reaction (PCR). Analysis revealed 120 genes were differentially expressed. Three genes were upregulated and 117 were downregulated (including interleukin [IL]-1β, IL-1R2, and IL-6R) in GCs of older versus younger patients. Our data provide evidence of downregulation in IL-1 and IL-6 gene families in luteinized GCs with advancing age. Given previously recognized roles for the IL gene family in folliculogenesis and ovulation, our findings may partly explain ovulatory and luteal dysfunctions associated with reproductive aging.

Original languageEnglish (US)
Pages (from-to)56-67
Number of pages12
JournalReproductive Sciences
Volume17
Issue number1
DOIs
StatePublished - Jan 1 2010

Keywords

  • Gene expression
  • Granulosa cells
  • Interleukin-1
  • Interleukin-6.
  • Reproductive aging

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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