Reproduction of human fibrous dysplasia of bone in immunocompromised mice by transplanted mosaics of normal and Gsα-mutated skeletal progenitor cells

Paolo Bianco, Sergei A. Kuznetsov, Mara Riminucci, Larry W. Fisher, Allen M. Spiegel, Pamela Gehron Robey

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

We have isolated progenitor cells from the stromal system of the fibrous dysplastic marrow of patients with McCune-Albright Syndrome. Analysis of the Gsα gene from individual colonies provided direct evidence for the presence of two different genotypes within single fibrous dysplastic lesions: marrow stromal cells containing two normal Gsα alleles, and those containing one normal allele and an allele with an activating mutation. Transplantation of clonal populations of normal cells into the subcutis of immunocompromised mice resulted in normal ossicle formation. In contrast, transplantation of clonal populations of mutant cells always led to the loss of transplanted cells from the transplantation site and no ossicle formation. However, transplantation of a mixture of normal and mutant cells reproduced an abnormal ectopic ossicle recapitulating human fibrous dysplasia and providing an in vivo cellular model of this disease. These results provide experimental evidence for the necessity of both normal and mutant cells in the development of McCune-Albright Syndrome fibrous dysplastic lesions in bone.

Original languageEnglish (US)
Pages (from-to)1737-1744
Number of pages8
JournalJournal of Clinical Investigation
Volume101
Issue number8
StatePublished - Apr 15 1998
Externally publishedYes

Fingerprint

Fibrous Dysplasia of Bone
Reproduction
Stem Cells
Polyostotic Fibrous Dysplasia
Transplantation
Alleles
Bone Marrow
Cell Transplantation
Stromal Cells
Population
Genotype
Bone and Bones
Mutation
Genes

Keywords

  • G protein
  • Marrow stromal cells
  • McCune-Albright Syndrome
  • Somatic mosaicism
  • Transplantation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Reproduction of human fibrous dysplasia of bone in immunocompromised mice by transplanted mosaics of normal and Gsα-mutated skeletal progenitor cells. / Bianco, Paolo; Kuznetsov, Sergei A.; Riminucci, Mara; Fisher, Larry W.; Spiegel, Allen M.; Robey, Pamela Gehron.

In: Journal of Clinical Investigation, Vol. 101, No. 8, 15.04.1998, p. 1737-1744.

Research output: Contribution to journalArticle

Bianco, Paolo ; Kuznetsov, Sergei A. ; Riminucci, Mara ; Fisher, Larry W. ; Spiegel, Allen M. ; Robey, Pamela Gehron. / Reproduction of human fibrous dysplasia of bone in immunocompromised mice by transplanted mosaics of normal and Gsα-mutated skeletal progenitor cells. In: Journal of Clinical Investigation. 1998 ; Vol. 101, No. 8. pp. 1737-1744.
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