Repression of ribosome and tRNA synthesis in secretion-defective cells is signaled by a novel branch of the cell integrity pathway

Yun Li, Robyn D. Moir, Indra K. Sethy-Coraci, Jonathan R. Warner, Ian M. Willis

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

The transcription of ribosomal DNA, ribosomal protein (RP) genes, and 5S and tRNA genes by RNA polymerases (PoIs) I, II, and III, respectively, is rapidly and coordinately repressed upon interruption of the secretory pathway in Saccharomyces cerevisiae. We find that repression of ribosome and tRNA synthesis in secretion-defective cells involves activation of the cell integrity pathway. Transcriptional repression requires the upstream components of this pathway, including the Wsc family of putative plasma membrane sensors and protein kinase C (PKC), but not the downstream Bck1- Mkk1/2-Slt2 mitogen-activated protein kinase cascade. These findings reveal a novel PKC effector pathway that controls more than 85% of nuclear transcription. It is proposed that the coordination of ribosome and tRNA synthesis with cell growth may be achieved, in part, by monitoring the turgor pressure of the cell.

Original languageEnglish (US)
Pages (from-to)3843-3851
Number of pages9
JournalMolecular and Cellular Biology
Volume20
Issue number11
DOIs
StatePublished - Jun 2000

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Transfer RNA
Ribosomes
Protein Kinase C
RNA Polymerase I
Ribosomal Proteins
Secretory Pathway
Mitogen-Activated Protein Kinase 1
Ribosomal DNA
Genes
Saccharomyces cerevisiae
Blood Proteins
Membrane Proteins
Cell Membrane
Pressure
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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Repression of ribosome and tRNA synthesis in secretion-defective cells is signaled by a novel branch of the cell integrity pathway. / Li, Yun; Moir, Robyn D.; Sethy-Coraci, Indra K.; Warner, Jonathan R.; Willis, Ian M.

In: Molecular and Cellular Biology, Vol. 20, No. 11, 06.2000, p. 3843-3851.

Research output: Contribution to journalArticle

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