Replication pattern and quinacrine fluorescence of the chromosomes of a CV-1-derived african green monkey cell line

R. J. Parker, P. W. Allderoice, O. J. Miller, Carl L. Schildkraut

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

When tritiated thymidine is incorporated into cells of a heteroploid CV-1-derived line near the end of the DNA synthetic period (S). the chromosomes are preferentially labeled in the centromeric region. After staining with quinacrine, the centromeric region of every chromosome shows minimal fluorescence, whereas the remainder of most chromosomes shows fairly bright fluorescence with distinctive banding patterns. In contrast to the end-of-S pattern, the distribution of grains over chromosomes labeled during the middle of S is consistent with random labeling. A marker chromosome with a long secondary constriction in the long arm near the centromere possesses the same labeling pattern, with late replication limited to the centromeric region and perhaps the adjacent region of the short arm. The secondary constriction region itself is not late replicating, and it fails to fluoresce after quinacrine staining.

Original languageEnglish (US)
Pages (from-to)93-106
Number of pages14
JournalCytogenetic and Genome Research
Volume12
Issue number2
DOIs
StatePublished - 1973

Fingerprint

Cercopithecus aethiops
Quinacrine
Chromosomes
Fluorescence
Cell Line
Constriction
Staining and Labeling
Centromere
S Phase
Genetic Markers
Thymidine
1,2-diamino-1,2-N,N'-carbonyl-1,2-dideoxyglucose hydrate
DNA

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Genetics(clinical)
  • Cell Biology

Cite this

Replication pattern and quinacrine fluorescence of the chromosomes of a CV-1-derived african green monkey cell line. / Parker, R. J.; Allderoice, P. W.; Miller, O. J.; Schildkraut, Carl L.

In: Cytogenetic and Genome Research, Vol. 12, No. 2, 1973, p. 93-106.

Research output: Contribution to journalArticle

@article{ed903c7f709841b18e95f3af7b3d3e21,
title = "Replication pattern and quinacrine fluorescence of the chromosomes of a CV-1-derived african green monkey cell line",
abstract = "When tritiated thymidine is incorporated into cells of a heteroploid CV-1-derived line near the end of the DNA synthetic period (S). the chromosomes are preferentially labeled in the centromeric region. After staining with quinacrine, the centromeric region of every chromosome shows minimal fluorescence, whereas the remainder of most chromosomes shows fairly bright fluorescence with distinctive banding patterns. In contrast to the end-of-S pattern, the distribution of grains over chromosomes labeled during the middle of S is consistent with random labeling. A marker chromosome with a long secondary constriction in the long arm near the centromere possesses the same labeling pattern, with late replication limited to the centromeric region and perhaps the adjacent region of the short arm. The secondary constriction region itself is not late replicating, and it fails to fluoresce after quinacrine staining.",
author = "Parker, {R. J.} and Allderoice, {P. W.} and Miller, {O. J.} and Schildkraut, {Carl L.}",
year = "1973",
doi = "10.1159/000130444",
language = "English (US)",
volume = "12",
pages = "93--106",
journal = "Cytogenetic and Genome Research",
issn = "1424-8581",
publisher = "S. Karger AG",
number = "2",

}

TY - JOUR

T1 - Replication pattern and quinacrine fluorescence of the chromosomes of a CV-1-derived african green monkey cell line

AU - Parker, R. J.

AU - Allderoice, P. W.

AU - Miller, O. J.

AU - Schildkraut, Carl L.

PY - 1973

Y1 - 1973

N2 - When tritiated thymidine is incorporated into cells of a heteroploid CV-1-derived line near the end of the DNA synthetic period (S). the chromosomes are preferentially labeled in the centromeric region. After staining with quinacrine, the centromeric region of every chromosome shows minimal fluorescence, whereas the remainder of most chromosomes shows fairly bright fluorescence with distinctive banding patterns. In contrast to the end-of-S pattern, the distribution of grains over chromosomes labeled during the middle of S is consistent with random labeling. A marker chromosome with a long secondary constriction in the long arm near the centromere possesses the same labeling pattern, with late replication limited to the centromeric region and perhaps the adjacent region of the short arm. The secondary constriction region itself is not late replicating, and it fails to fluoresce after quinacrine staining.

AB - When tritiated thymidine is incorporated into cells of a heteroploid CV-1-derived line near the end of the DNA synthetic period (S). the chromosomes are preferentially labeled in the centromeric region. After staining with quinacrine, the centromeric region of every chromosome shows minimal fluorescence, whereas the remainder of most chromosomes shows fairly bright fluorescence with distinctive banding patterns. In contrast to the end-of-S pattern, the distribution of grains over chromosomes labeled during the middle of S is consistent with random labeling. A marker chromosome with a long secondary constriction in the long arm near the centromere possesses the same labeling pattern, with late replication limited to the centromeric region and perhaps the adjacent region of the short arm. The secondary constriction region itself is not late replicating, and it fails to fluoresce after quinacrine staining.

UR - http://www.scopus.com/inward/record.url?scp=0015796086&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015796086&partnerID=8YFLogxK

U2 - 10.1159/000130444

DO - 10.1159/000130444

M3 - Article

VL - 12

SP - 93

EP - 106

JO - Cytogenetic and Genome Research

JF - Cytogenetic and Genome Research

SN - 1424-8581

IS - 2

ER -