Replication of proto-oncogenes early during the S phase in mammalian cell lines

Mohammed A. Iqbal, Jeffrey Chinsky, Valerie Didamo, Carl L. Schildkraut

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Members of several classes of proto-oncogenes replicate during the first third of S-phase in two human (K562 erythroleukemia and HeLa), one Chinese hamster (CHO) and eight mouse cell lines. These cell lines exhibit a variety of specialized functions characteristic of pre-B and B cells, T cells and erythroid cells. The proto-oncogenes studied include fos, myc, myb, abl, fes, fms, mos, raf, rel, sis, Ha-ras, Ki-ras, and N-ras. In K562 cells, amplified and rearranged c-abl genes show a pattern of temporal replication during S that is similar to the pattern observed for the 5′ breakpoint cluster region (bcr) and the amplified Cλ light chain immunoglobulin genes. The c-Ki-ras related sequences in CHO cells provide one example of late replicating proto-oncogene sequences that are present in multiple copies. The cellular gene N-myc replicates late during S in some of these cell lines. In three pre-B cell lines in which N-myc specific transcripts have been detected, N-myc replicates earlier in the S phase than in the other cell lines studied here.

Original languageEnglish (US)
Pages (from-to)87-103
Number of pages17
JournalNucleic Acids Research
Volume15
Issue number1
DOIs
StatePublished - Jan 12 1987

Fingerprint

Proto-Oncogenes
S Phase
Replication
Cells
Cell Line
Line
Cell
Genes
B Cells
Immunoglobulin Light Chain Genes
abl Genes
Gene
Leukemia, Erythroblastic, Acute
Erythroid Cells
myc Genes
B-Lymphoid Precursor Cells
K562 Cells
CHO Cells
Immunoglobulin Light Chains
Cricetulus

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Replication of proto-oncogenes early during the S phase in mammalian cell lines. / Iqbal, Mohammed A.; Chinsky, Jeffrey; Didamo, Valerie; Schildkraut, Carl L.

In: Nucleic Acids Research, Vol. 15, No. 1, 12.01.1987, p. 87-103.

Research output: Contribution to journalArticle

Iqbal, Mohammed A. ; Chinsky, Jeffrey ; Didamo, Valerie ; Schildkraut, Carl L. / Replication of proto-oncogenes early during the S phase in mammalian cell lines. In: Nucleic Acids Research. 1987 ; Vol. 15, No. 1. pp. 87-103.
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