Replication and persistence of HPV DNA in cultured cells derived from laryngeal papillomas

Teresa P. DiLorenzo, Lorne B. Taichman, Bettie M. Steinberg

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We have investigated the replication and persistence of human papillomavirus (HPV) type 6 and 11 DNA in cultured cells derived from laryngeal papillomas, with paradoxical findings. Measured by bromodeoxyuridine incorporation into heavy/light DNA separated on a cesium chloride gradient, viral DNA replicates in both primary and secondary cells. The ratio of the fraction of replicated viral to replicated cellular DNA was equal to or greater than 1 in all but one case and was closer to 2 in primary cells. Despite this efficient replication, HPV DNA is rapidly lost from the cells with passage. We propose that infected cells, or those with a high HPV copy number, show a selective decrease in plating efficiency compared to uninfected cells or those with a low copy number, which explains the loss of HPV DNA with repeated passage.

Original languageEnglish (US)
Pages (from-to)148-153
Number of pages6
JournalVirology
Volume186
Issue number1
DOIs
StatePublished - 1992

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Papilloma
Cultured Cells
DNA
Human papillomavirus 11
Human papillomavirus 6
Viral DNA
Bromodeoxyuridine
Light

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Replication and persistence of HPV DNA in cultured cells derived from laryngeal papillomas. / DiLorenzo, Teresa P.; Taichman, Lorne B.; Steinberg, Bettie M.

In: Virology, Vol. 186, No. 1, 1992, p. 148-153.

Research output: Contribution to journalArticle

DiLorenzo, Teresa P. ; Taichman, Lorne B. ; Steinberg, Bettie M. / Replication and persistence of HPV DNA in cultured cells derived from laryngeal papillomas. In: Virology. 1992 ; Vol. 186, No. 1. pp. 148-153.
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