Replication and persistence of HPV DNA in cultured cells derived from laryngeal papillomas

Teresa P. DiLorenzo, Lorne B. Taichman, Bettie M. Steinberg

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We have investigated the replication and persistence of human papillomavirus (HPV) type 6 and 11 DNA in cultured cells derived from laryngeal papillomas, with paradoxical findings. Measured by bromodeoxyuridine incorporation into heavy/light DNA separated on a cesium chloride gradient, viral DNA replicates in both primary and secondary cells. The ratio of the fraction of replicated viral to replicated cellular DNA was equal to or greater than 1 in all but one case and was closer to 2 in primary cells. Despite this efficient replication, HPV DNA is rapidly lost from the cells with passage. We propose that infected cells, or those with a high HPV copy number, show a selective decrease in plating efficiency compared to uninfected cells or those with a low copy number, which explains the loss of HPV DNA with repeated passage.

Original languageEnglish (US)
Pages (from-to)148-153
Number of pages6
JournalVirology
Volume186
Issue number1
DOIs
Publication statusPublished - Jan 1992

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ASJC Scopus subject areas

  • Virology

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