Replacement of liver function in rats by transplantation of microcarrier-attached hepatocytes

Achilles A. Demetriou, James F. Whiting, David Feldman, Stanley M. Levenson, Namita Roy Chowdhury, Albert D. Moscioni, Michael Kram, Jayanta Roy-Chowdhury

Research output: Contribution to journalArticle

240 Citations (Scopus)

Abstract

Isolated hepatocytes, harvested from normal rat livers by portal vein collagenase perfusion, can be attached to collagen-coated dextran microcarriers and transplanted by intraperitoneal injection into rats. Survival and function of the transplanted hepatocytes have been demonstrated in mutant rats lacking bilirubin-uridine diphosphate glucuronosyltransferase activity (Gunn strain) and rats with inherited lack of plasma albumin (Nagase analbuminemia rat strain). This simple technique promises to be useful in the treatment of acute liver failure in humans.

Original languageEnglish (US)
Pages (from-to)1190-1192
Number of pages3
JournalScience
Volume233
Issue number4769
StatePublished - 1986

Fingerprint

Hepatocytes
Transplantation
Liver
Gunn Rats
Acetylglucosaminidase
Glucuronosyltransferase
Uridine Diphosphate
Acute Liver Failure
Collagenases
Portal Vein
Dextrans
Intraperitoneal Injections
Bilirubin
Serum Albumin
Collagen
Perfusion
Survival
Therapeutics

ASJC Scopus subject areas

  • General

Cite this

Demetriou, A. A., Whiting, J. F., Feldman, D., Levenson, S. M., Chowdhury, N. R., Moscioni, A. D., ... Roy-Chowdhury, J. (1986). Replacement of liver function in rats by transplantation of microcarrier-attached hepatocytes. Science, 233(4769), 1190-1192.

Replacement of liver function in rats by transplantation of microcarrier-attached hepatocytes. / Demetriou, Achilles A.; Whiting, James F.; Feldman, David; Levenson, Stanley M.; Chowdhury, Namita Roy; Moscioni, Albert D.; Kram, Michael; Roy-Chowdhury, Jayanta.

In: Science, Vol. 233, No. 4769, 1986, p. 1190-1192.

Research output: Contribution to journalArticle

Demetriou, AA, Whiting, JF, Feldman, D, Levenson, SM, Chowdhury, NR, Moscioni, AD, Kram, M & Roy-Chowdhury, J 1986, 'Replacement of liver function in rats by transplantation of microcarrier-attached hepatocytes', Science, vol. 233, no. 4769, pp. 1190-1192.
Demetriou AA, Whiting JF, Feldman D, Levenson SM, Chowdhury NR, Moscioni AD et al. Replacement of liver function in rats by transplantation of microcarrier-attached hepatocytes. Science. 1986;233(4769):1190-1192.
Demetriou, Achilles A. ; Whiting, James F. ; Feldman, David ; Levenson, Stanley M. ; Chowdhury, Namita Roy ; Moscioni, Albert D. ; Kram, Michael ; Roy-Chowdhury, Jayanta. / Replacement of liver function in rats by transplantation of microcarrier-attached hepatocytes. In: Science. 1986 ; Vol. 233, No. 4769. pp. 1190-1192.
@article{2c19b85d96f2404581f65cc86059d58c,
title = "Replacement of liver function in rats by transplantation of microcarrier-attached hepatocytes",
abstract = "Isolated hepatocytes, harvested from normal rat livers by portal vein collagenase perfusion, can be attached to collagen-coated dextran microcarriers and transplanted by intraperitoneal injection into rats. Survival and function of the transplanted hepatocytes have been demonstrated in mutant rats lacking bilirubin-uridine diphosphate glucuronosyltransferase activity (Gunn strain) and rats with inherited lack of plasma albumin (Nagase analbuminemia rat strain). This simple technique promises to be useful in the treatment of acute liver failure in humans.",
author = "Demetriou, {Achilles A.} and Whiting, {James F.} and David Feldman and Levenson, {Stanley M.} and Chowdhury, {Namita Roy} and Moscioni, {Albert D.} and Michael Kram and Jayanta Roy-Chowdhury",
year = "1986",
language = "English (US)",
volume = "233",
pages = "1190--1192",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "4769",

}

TY - JOUR

T1 - Replacement of liver function in rats by transplantation of microcarrier-attached hepatocytes

AU - Demetriou, Achilles A.

AU - Whiting, James F.

AU - Feldman, David

AU - Levenson, Stanley M.

AU - Chowdhury, Namita Roy

AU - Moscioni, Albert D.

AU - Kram, Michael

AU - Roy-Chowdhury, Jayanta

PY - 1986

Y1 - 1986

N2 - Isolated hepatocytes, harvested from normal rat livers by portal vein collagenase perfusion, can be attached to collagen-coated dextran microcarriers and transplanted by intraperitoneal injection into rats. Survival and function of the transplanted hepatocytes have been demonstrated in mutant rats lacking bilirubin-uridine diphosphate glucuronosyltransferase activity (Gunn strain) and rats with inherited lack of plasma albumin (Nagase analbuminemia rat strain). This simple technique promises to be useful in the treatment of acute liver failure in humans.

AB - Isolated hepatocytes, harvested from normal rat livers by portal vein collagenase perfusion, can be attached to collagen-coated dextran microcarriers and transplanted by intraperitoneal injection into rats. Survival and function of the transplanted hepatocytes have been demonstrated in mutant rats lacking bilirubin-uridine diphosphate glucuronosyltransferase activity (Gunn strain) and rats with inherited lack of plasma albumin (Nagase analbuminemia rat strain). This simple technique promises to be useful in the treatment of acute liver failure in humans.

UR - http://www.scopus.com/inward/record.url?scp=0022460518&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022460518&partnerID=8YFLogxK

M3 - Article

VL - 233

SP - 1190

EP - 1192

JO - Science

JF - Science

SN - 0036-8075

IS - 4769

ER -