Remodeling of the sarcomeric cytoskeleton in cardiac ventricular myocytes during heart failure and after cardiac resynchronization therapy

Justin G. Lichter, Eric Carruth, Chelsea Mitchell, Andreas S. Barth, Takeshi Aiba, David A. Kass, Gordon F. Tomaselli, John H. Bridge, Frank B. Sachse

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Sarcomeres are the basic contractile units of cardiac myocytes. Recent studies demonstrated remodeling of sarcomeric proteins in several diseases, including genetic defects and heart failure. Here we investigated remodeling of sarcomeric α-actinin in two models of heart failure, synchronous (SHF) and dyssynchronous heart failure (DHF), as well as a model of cardiac resynchronization therapy (CRT). We applied three-dimensional confocal microscopy and quantitative methods of image analysis to study isolated cells from our animal models. 3D Fourier analysis revealed a decrease of the spatial regularity of the α-actinin distribution in both SHF and DHF versus control cells. The spatial regularity of α-actinin in DHF cells was reduced when compared with SHF cells. The spatial regularity of α-actinin was partially restored after CRT. We found longitudinal depositions of α-actinin in SHF, DHF and CRT cells. These depositions spanned adjacent Z-disks and exhibited a lower density of α-actinin than in the Z-disk. Differences in the occurrence of depositions between the SHF, CRT and DHF models versus control were significant. Also, CRT cells exhibited a higher occurrence of depositions versus SHF, but not DHF cells. Other sarcomeric proteins did not accumulate in the depositions to the same extent as α-actinin. We did not find differences in the expression of α-actinin protein and its encoding gene in our animal models. In summary, our studies indicate that HF is associated with two different types of remodeling of α-actinin and only one of those was reversed after CRT. We suggest that these results can guide us to an understanding of remodeling of structures and function associated with sarcomeres.

Original languageEnglish (US)
Pages (from-to)86-95
Number of pages10
JournalJournal of Molecular and Cellular Cardiology
Volume72
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Actinin
Cardiac Resynchronization Therapy
Cytoskeleton
Cardiac Myocytes
Heart Failure
Sarcomeres
Animal Models
Inborn Genetic Diseases
Proteins
Fourier Analysis
Confocal Microscopy

Keywords

  • Alpha actinin
  • Cardiac recovery
  • Cardiac resynchronization therapy
  • Heart failure
  • Remodeling

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Remodeling of the sarcomeric cytoskeleton in cardiac ventricular myocytes during heart failure and after cardiac resynchronization therapy. / Lichter, Justin G.; Carruth, Eric; Mitchell, Chelsea; Barth, Andreas S.; Aiba, Takeshi; Kass, David A.; Tomaselli, Gordon F.; Bridge, John H.; Sachse, Frank B.

In: Journal of Molecular and Cellular Cardiology, Vol. 72, 01.01.2014, p. 86-95.

Research output: Contribution to journalArticle

Lichter, Justin G. ; Carruth, Eric ; Mitchell, Chelsea ; Barth, Andreas S. ; Aiba, Takeshi ; Kass, David A. ; Tomaselli, Gordon F. ; Bridge, John H. ; Sachse, Frank B. / Remodeling of the sarcomeric cytoskeleton in cardiac ventricular myocytes during heart failure and after cardiac resynchronization therapy. In: Journal of Molecular and Cellular Cardiology. 2014 ; Vol. 72. pp. 86-95.
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