Release of guanosine triphosphate binding protein a subunits from mouse myocardial membranes

Basic properties and their alterations in acute murine Chagas disease

Huan Huang, Murray Wittner, Herbert Tanowitz, John P. Bilezikian, Stephen A. Morris

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: The aim was to investigate the arrangement of heterotrimeric αβγ G proteins in myocardial membranes using GTPγS dependent release characteristics of their α subunits in acute murine Chagas disease. Methods: The properties of GTP-γS dependent α subunit release were monitored immunochemically as well as by cholera toxin and pertussis toxin catalysed [32P]ADP ribosylation. Results: GTPγS, as opposed to other nucleotides, caused optimal and virtually instantaneous release of soluble 40 kDa [32P]ADP ribosylated protein in pertussis toxin treated membranes. When determined immunochemically, infection decreased both the sensitivity to GTP-γS dependent release of αi3 subunits and appeared to facilitate the appearance of GTPγS dependent release of αi3. GTP-γS also caused the release of soluble 45 and 40 kDa proteins as detected by cholera toxin-[32P]ADP ribosylated membranes and immunochemical analysis. With regard to cholera toxin-[32P]ADP ribosylated Gs substrates sensitive to GTP-γS dependent release, infection (1) decreased the amount of 45 kDa as protein, (2) increased the amount of 40 kDa protein, and (3) enhanced sensitivity to GTPγS. In contrast, there was no effect of infection on the magnitude or sensitivity to GTPγS dependent release of immunochemical as. Conclusions: The diverse characteristics of GTPγS dependent release of the very similar α subunits from myocardial membranes and their unique sensitivity to infection with T cruzi suggest that these very similar proteins are arranged within the plasma membrane in such a manner as to modify their biochemical behaviour.

Original languageEnglish (US)
Pages (from-to)350-358
Number of pages9
JournalCardiovascular Research
Volume29
Issue number3
StatePublished - 1995

Fingerprint

Chagas Disease
Protein Subunits
Guanosine Triphosphate
Carrier Proteins
Adenosine Diphosphate
Cholera Toxin
Membranes
Pertussis Toxin
Infection
Proteins
Heterotrimeric GTP-Binding Proteins
Nucleotides
Cell Membrane

Keywords

  • GTP binding proteins: Murine chagas disease
  • GTPγS

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Release of guanosine triphosphate binding protein a subunits from mouse myocardial membranes : Basic properties and their alterations in acute murine Chagas disease. / Huang, Huan; Wittner, Murray; Tanowitz, Herbert; Bilezikian, John P.; Morris, Stephen A.

In: Cardiovascular Research, Vol. 29, No. 3, 1995, p. 350-358.

Research output: Contribution to journalArticle

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