Release of guanosine triphosphate binding protein α subunits from mouse myocardial membranes: Basic properties and their alterations in acute murine chagas disease

Huan Huang, Murray Wittner, Herbert Tanowitz, John P. Bilezikian, Stephen A. Morris

Research output: Contribution to journalArticle

Abstract

Objective: The aim was to investigate the arrangement of heterotrimeric αβγ G proteins in myocardial membranes using GTPγS dependent release characteristics of their α subunits in acute murine Chagas disease. Methods: The properties of GTPγS dependent α subunit release were monitored immunochemically as well as by cholera toxin and pertussis toxin catalysed [32P]ADP ribosylation. Results: GTPγS, as opposed to other nucleotides, caused optimal and virtually instantaneous release of soluble 40 kDa [32P]ADP ribosylated protein in pertussis toxin treated membranes. When determined immunochemically, infection decreased both the sensitivity to GTPγS dependent release of αi subunits and appeared to facilitate the appearance of GTPγS dependent release of αi3. GTPγS also caused the release of soluble 45 and 40 kDa proteins as detected by cholera toxin-[32P]ADP ribosylated membranes and immunochemical analysis. With regard to cholera toxin-[32P]ADP ribosylated Gs substrates sensitive to GTPγS dependent release, infection (1) decreased the amount of 45 kDa as protein, (2) increased the amount of 40 kDa protein, and (3) enhanced sensitivity to GTPγS. In contrast, there was no effect of infection on the magnitude or sensitivity to GTPγS dependent release of immunochemical αS. Conclusions: The diverse characteristics of GTPγS dependent release of the very similar α subunits from myocardial membranes and their unique sensitivity to infection with T cruzi. suggest that these very similar proteins are arranged within the plasma membrane in such a manner as to modify their biochemical behaviour.

Original languageEnglish (US)
Pages (from-to)350-358
Number of pages9
JournalCardiovascular Research
Volume28
Issue number9
DOIs
StatePublished - Sep 1994

Fingerprint

Chagas Disease
Protein Subunits
Guanosine Triphosphate
Acute
Administrative data processing
Mouse
Carrier Proteins
Membrane
Adenosine Diphosphate
Cholera Toxin
Proteins
Membranes
Protein
Dependent
Infection
Pertussis Toxin
Heterotrimeric GTP-Binding Proteins
G Protein
Cell membranes
Nucleotides

Keywords

  • GTP binding proteins
  • GTPγS
  • Murine Chagas disease

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Release of guanosine triphosphate binding protein α subunits from mouse myocardial membranes : Basic properties and their alterations in acute murine chagas disease. / Huang, Huan; Wittner, Murray; Tanowitz, Herbert; Bilezikian, John P.; Morris, Stephen A.

In: Cardiovascular Research, Vol. 28, No. 9, 09.1994, p. 350-358.

Research output: Contribution to journalArticle

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