Relationship between the electron density of the heme Fe atom and the vibrational frequencies of the fe-bound carbon monoxide in myoglobin

Ryu Nishimura, Tomokazu Shibata, Hulin Tai, Izumi Ishigami, Takashi Ogura, Satoshi Nagao, Takashi Matsuo, Shun Hirota, Kiyohiro Imai, Saburo Neya, Akihiro Suzuki, Yasuhiko Yamamoto

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

We analyzed the vibrational frequencies of the Fe-bound carbon monoxide (CO) of myoglobin reconstituted with a series of chemically modified heme cofactors possessing a heme Fe atom with a variety of electron densities. The study revealed that the stretching frequency of Fe-bound CO (νCO) increases with decreasing electron density of the heme Fe atom (ρFe). This finding demonstrated that the νCO value can be used as a sensitive measure of the ρFe value and that the π back-donation of the heme Fe atom to CO is affected by the heme π-system perturbation induced through peripheral side chain modifications.

Original languageEnglish (US)
Pages (from-to)3349-3355
Number of pages7
JournalInorganic Chemistry
Volume52
Issue number6
DOIs
Publication statusPublished - Mar 18 2013
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Inorganic Chemistry

Cite this