Regulatory mutations in CHO cells induce expression of the mouse embryonic antigen SSEA-1

Christine Campbell, Pamela Stanley

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Two rare and dominant mutants of Chinese hamster ovary (CHO) cells, LEC11 and LEC12, express the mouse embryonic antigen SSEA-1. Parental CHO cells and the revertants, LEC11.R9 and LEC12.R10, do not express this antigen as detected by a sensitive radioimmunoassay with a monoclonal antibody to SSEA-1. The presence of the SSEA-1 determinant correlates with the apparent de novo expression of specific N-acetylglucosaminide α(1,3)fucosyl-transferase activities not detected in parental or revertant cell extracts. Several differences in the enzymes substrate specificities and their products have been identified. The combined data suggest that LEC11 and LEC12 mutants result from regulatory mutations affecting different fucosyltransferase genes.

Original languageEnglish (US)
Pages (from-to)303-309
Number of pages7
JournalCell
Volume35
Issue number1
DOIs
StatePublished - Nov 1983

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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