TY - JOUR
T1 - Regulatory mutations in CHO cells induce expression of the mouse embryonic antigen SSEA-1
AU - Campbell, Christine
AU - Stanley, Pamela
N1 - Funding Information:
The authors thank Grace Vivona for excellent technical assistance, Dr . Davor Solter (Wistar Institute) for anti-SSEA-1 monoclonal antibody, Dr . Tom Beyer (Duke University) for 2'-fucosyllactose, Dr. Petr Draber (Institute of Molecular Genetics, Czechoslovakia) for preparing and determining the binding of ' n l-anti-SSEA-1 to 3T6 and F9 cells (Table 1), and Drs . Tony Adamany and Matthew Scharff (Albert Einstein College of Medicine) for helpful discussions . This work was supported by a grant (BC322B) from the American Cancer Society . Additional support was provided by PHS grant 1 -R01 -CA306 5, awarded by the National Cancer Institute, DHHS, and the AECOM Core Cancer Grant (NIH 1P01 1 CA132330) . C . E . C . is the recipient of a fellowship from the Medical Research Council of Canada . P . S . is the recipient of a faculty award from the American Cancer Society .
PY - 1983/11
Y1 - 1983/11
N2 - Two rare and dominant mutants of Chinese hamster ovary (CHO) cells, LEC11 and LEC12, express the mouse embryonic antigen SSEA-1. Parental CHO cells and the revertants, LEC11.R9 and LEC12.R10, do not express this antigen as detected by a sensitive radioimmunoassay with a monoclonal antibody to SSEA-1. The presence of the SSEA-1 determinant correlates with the apparent de novo expression of specific N-acetylglucosaminide α(1,3)fucosyl-transferase activities not detected in parental or revertant cell extracts. Several differences in the enzymes substrate specificities and their products have been identified. The combined data suggest that LEC11 and LEC12 mutants result from regulatory mutations affecting different fucosyltransferase genes.
AB - Two rare and dominant mutants of Chinese hamster ovary (CHO) cells, LEC11 and LEC12, express the mouse embryonic antigen SSEA-1. Parental CHO cells and the revertants, LEC11.R9 and LEC12.R10, do not express this antigen as detected by a sensitive radioimmunoassay with a monoclonal antibody to SSEA-1. The presence of the SSEA-1 determinant correlates with the apparent de novo expression of specific N-acetylglucosaminide α(1,3)fucosyl-transferase activities not detected in parental or revertant cell extracts. Several differences in the enzymes substrate specificities and their products have been identified. The combined data suggest that LEC11 and LEC12 mutants result from regulatory mutations affecting different fucosyltransferase genes.
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U2 - 10.1016/0092-8674(83)90233-7
DO - 10.1016/0092-8674(83)90233-7
M3 - Article
C2 - 6138159
AN - SCOPUS:0021074339
SN - 0092-8674
VL - 35
SP - 303
EP - 309
JO - Cell
JF - Cell
IS - 1
ER -